Yao Cheng-can, Li Xiao-kun, Huang Yao-xiong
College of Pharmacy, Jinan University, Guangzhou, China.
Guang Pu Xue Yu Guang Pu Fen Xi. 2005 Apr;25(4):613-6.
The instant effect of temperature on the absorption spectra of the hemoglobin in single living intact red blood cells was investigated, by employing a highly sensitive fast multi-channel micro-spectrophotometer system to perform non-invasive, in situ, real time measurements on the cells. It was found that both the heights and position of the specific peaks in the absorption spectra of intercellular hemoglobin were changed with temperature, indicating that the oxygen carrying capacity of red blood cells varies with temperature. The correlations of the structure and concentration as well as the function of hemoglobin, and the molecular mechanism were also discussed.
利用高灵敏度快速多通道显微分光光度计系统对单个活的完整红细胞内血红蛋白的吸收光谱进行非侵入性、原位、实时测量，研究了温度对其的即时影响。结果发现，细胞内血红蛋白吸收光谱中特定峰的高度和位置均随温度变化，这表明红细胞的携氧能力随温度而变化。文中还讨论了血红蛋白的结构、浓度与功能之间的相关性以及分子机制。
