Benincasa Maria-Anna, Moore Lee R, Williams P Stephen, Poptic Earl, Carpino Francesca, Zborowski Maciej
Department of Chemistry, University of Rome La Sapienza, Piazzale A. Moro 5, 00185 Rome, Italy.
Anal Chem. 2005 Aug 15;77(16):5294-301. doi: 10.1021/ac058013o.
The need for innovative separative techniques suitable for the fractionation of biomaterials prompted this investigation into the performance of the gravitational split-flow thin channel (G-SPLITT) system as a cell sorter. The rigorous mathematical description of the separation mechanism allows achievement of fast separation of several million myeloma cells from healthy splenocytes using flow conditions calculated from theory. Separation in G-SPLITT is based on differences in sedimentation rate. For accurate prediction of the optimal working conditions, this parameter was directly measured by cell tracking velocimetry rather than relying on a measure of diameter (by Multisizer) and an assumed density for each cell population. We also discuss the influence of different flow conditions on the effectiveness of separation.
对适用于生物材料分级分离的创新分离技术的需求促使了对重力分流薄通道（G-SPLITT）系统作为细胞分选器性能的这项研究。分离机制的严格数学描述使得能够利用从理论计算得出的流动条件，快速从健康脾细胞中分离出数百万个骨髓瘤细胞。G-SPLITT中的分离基于沉降速率的差异。为了准确预测最佳工作条件，该参数通过细胞跟踪测速法直接测量，而不是依赖于（通过库尔特计数器）测量直径以及为每个细胞群体假定的密度。我们还讨论了不同流动条件对分离效果的影响。
