Schermann Sonya M, Simmons Douglas A, Konermann Lars
Department of Biochemistry, University of Oxford, South Parks Road, Oxford, OX1 3QU, UK.
Expert Rev Proteomics. 2005 Aug;2(4):475-85. doi: 10.1586/14789450.2.4.475.
One of the greatest current challenges in proteomics is to develop an understanding of cellular communication and regulation processes, most of which involve noncovalent interactions of proteins with various binding partners. Mass spectrometry plays an important role in all aspects of these research efforts. This article provides a survey of mass spectrometry-based approaches for exploring protein-ligand interactions. A wide array of techniques is available, and the choice of method depends on the specific problem at hand. For example, the high-throughput screening of compound libraries for binding to a specific receptor requires different approaches than structural studies on multiprotein complexes. This review is directed to readers wishing to obtain a concise yet comprehensive overview of existing experimental techniques. Specific emphasis is placed on emerging methods that have been developed within the last few years.
蛋白质组学当前面临的最大挑战之一是深入了解细胞通讯和调控过程，其中大部分涉及蛋白质与各种结合伴侣的非共价相互作用。质谱在这些研究工作的各个方面都发挥着重要作用。本文综述了基于质谱的探索蛋白质-配体相互作用的方法。有各种各样的技术可供选择，方法的选择取决于手头的具体问题。例如，对化合物库与特定受体结合进行高通量筛选所需的方法与对多蛋白复合物进行结构研究所需的方法不同。这篇综述针对的是希望获得现有实验技术简明而全面概述的读者。特别强调了过去几年中开发的新兴方法。
