Fejgin Moshe D, Pomeranz Meir, Liberman Meytal, Fishman Ami, Amiel Aliza
Department of Obstetrics and Gynecology, Meir Hospital, Sackler School of Medicine, Kfar-Saba 44281, Israel.
Acta Obstet Gynecol Scand. 2005 Sep;84(9):860-3. doi: 10.1111/j.0001-6349.2005.00757.x.
In this study, we applied the fluorescent in situ hybridization (FISH) technique and compared the common numerical abnormalities with chromosomes 13, 16, 18, 21, X, and Y in spontaneous to artificial abortion. This would cover about 75% of the common aneuploidy in spontaneous abortion.
Placentas were taken from 59 patients with a first trimester spontaneous abortion and 61 patients who underwent an elective first trimester pregnancy termination. The range of growth was from 5 to 12 gestational weeks. Placentas were processed according to direct chorionic villi preparation. Direct dual color FISH was performed according to Vysis protocol with the probes for the following chromosomes: 13, 16, 18, 21, X, and Y.
The aneuploidy rate in spontaneous abortion was 55.9% and in artificial abortion 8.2%. There was a significant difference between the two groups in the aneuploidy rate (P = 6 x 10(-9)).
FISH is a rapid, efficient, and relatively inexpensive tool in detecting aneuploidy in placentas from cases of spontaneous abortions. Our rate of detected aneuploidy is compatible with other reports in which conventional cytogenetics was utilized.
在本研究中，我们应用荧光原位杂交（FISH）技术，比较自然流产与人工流产中13、16、18、21、X和Y染色体常见的数目异常情况。这将涵盖自然流产中约75%的常见非整倍体情况。
取自59例孕早期自然流产患者和61例接受选择性孕早期终止妊娠患者的胎盘。孕周范围为5至12周。胎盘按照直接绒毛膜绒毛制备方法进行处理。根据Vysis方案，使用针对以下染色体的探针进行直接双色FISH检测：13、16、18、21、X和Y。
自然流产中的非整倍体率为55.9%，人工流产中的为8.2%。两组之间的非整倍体率存在显著差异（P = 6×10⁻⁹）。
FISH是检测自然流产病例胎盘中非整倍体的一种快速、高效且相对廉价的工具。我们检测到的非整倍体率与使用传统细胞遗传学的其他报告结果相符。
