Li Wei-guo, Ai Hong, Chen Wei-liang, Jiang Li-jian, Wu Xin-zhong
Department of Stomatology, Third Affiliated Hospital of Sun Yat-sen University, Guangzhou 510630, China.
Chin Med J (Engl). 2005 Jul 5;118(13):1099-104.
Although 32P-glass microspheres (32P-GMS) have been used in internal radiotherapy for malignant tumors, it has been one of the key obstacles to improve the effect of radiotherapy. We investigated the cellular and hypersensitive effect of combined use of low dose of cisplatin and interstitial injection of 32P-GMS on mouse solid tumor S180.
The mice with solid tumor S180 were randomly divided into four groups (controls, cisplatin therapy, 32P-GMS therapy and combination therapy). The specimens of the mice were sectioned two weeks after treatment and weighed. The death rate of tumor cells and the inhibition rate of tumor were calculated respectively. The cell cycle and apoptosis rate were evaluated with flow-cytometry. The ultrastructural changes of the four groups were observed by a transmission electron microscope. The data were analyzed by the chi-square test.
The growth of tumor was slower in the combination therapy group than in the simple therapy groups by macrography. The inhibition rate and the death rate of tumor cells of the combination therapy group were significantly higher than those of the control group and the other two simple therapy groups (P < 0.05). More cell damages were displayed in the combination therapy group than in the other groups under the light and electronic microscope.
Low-dose cisplatin combined with interstitial injection of 32P glass microspheres could be used as an effective hypersensitive regimen for the internal radiotherapy of mouse solid tumor S180.
尽管32P玻璃微球（32P-GMS）已用于恶性肿瘤的内放射治疗，但提高放疗效果一直是关键障碍之一。我们研究了低剂量顺铂联合32P-GMS间质注射对小鼠实体瘤S180的细胞和增敏作用。
将荷S180实体瘤小鼠随机分为四组（对照组、顺铂治疗组、32P-GMS治疗组和联合治疗组）。治疗两周后将小鼠标本切片并称重。分别计算肿瘤细胞死亡率和肿瘤抑制率。采用流式细胞术评估细胞周期和凋亡率。用透射电子显微镜观察四组的超微结构变化。数据采用卡方检验进行分析。
大体观察显示联合治疗组肿瘤生长比单纯治疗组慢。联合治疗组肿瘤细胞抑制率和死亡率显著高于对照组及其他两个单纯治疗组（P<0.05）。光镜和电镜下联合治疗组比其他组显示出更多的细胞损伤。
低剂量顺铂联合32P玻璃微球间质注射可作为小鼠实体瘤S180内放射治疗的有效增敏方案。
