LuxS/自诱导物-2群体感应分子调控艰难梭菌中致病基因的转录表达。
LuxS/autoinducer-2 quorum sensing molecule regulates transcriptional virulence gene expression in Clostridium difficile.
作者信息
Lee Alex Sek Yew, Song Keang Peng
机构信息
Microbial Pathogenesis Laboratory, Department of Microbiology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore.
出版信息
Biochem Biophys Res Commun. 2005 Sep 30;335(3):659-66. doi: 10.1016/j.bbrc.2005.07.131.
Toxigenic Clostridium difficile CCUG19126 produces the autoinducer-2 (AI-2) quorum sensing molecule that induces bioluminescence in Vibrio harveyi BB170 reporter strain. AI-2-containing cell-free supernatants from mid-log phase C. difficile and Escherichia coli DH5alpha expressing recombinant luxS(cd) upregulated the transcript levels of tcdA (7-10-fold), tcdB (4-6-fold), and tcdE (2-3-fold) in early-log C. difficile. In contrast, no induction occurred when cells were exposed to sterile medium or to cell-free supernatant from E. coli DH5alpha. AI-2 did not significantly increase the level of toxin A in early-log C. difficile. These results suggest that LuxS(cd)-dependent signaling regulates virulence gene expression at the transcriptional level in C. difficile.
产毒艰难梭菌CCUG19126产生自诱导物-2（AI-2）群体感应分子，该分子可诱导哈维弧菌BB170报告菌株发出生物荧光。来自对数中期艰难梭菌和表达重组luxS（cd）的大肠杆菌DH5α的含AI-2无细胞上清液，上调了对数早期艰难梭菌中tcdA（7至10倍）、tcdB（4至6倍）和tcdE（2至3倍）的转录水平。相比之下，当细胞暴露于无菌培养基或大肠杆菌DH5α的无细胞上清液时，未发生诱导。AI-2并未显著增加对数早期艰难梭菌中毒素A的水平。这些结果表明，依赖LuxS（cd）的信号传导在转录水平上调节艰难梭菌的毒力基因表达。
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