Nägele Edgar, Moritz Ralf
Agilent Technologies R&D and Marketing GmbH and Company, Waldbronn, Germany.
J Am Soc Mass Spectrom. 2005 Oct;16(10):1670-6. doi: 10.1016/j.jasms.2005.06.002.
Today, it is necessary to identify relevant compounds appearing in discovery and development of new drug substances in the pharmaceutical industry. For that purpose, the measurement of accurate molecular mass and empirical formula calculation is very important for structure elucidation in addition to other available analytical methods. In this work, the identification and confirmation of degradation products in a finished dosage form of the antibiotic drug amoxicillin obtained under stress conditions will be demonstrated. Structure elucidation is performed utilizing liquid chromatography (LC) ion trap MS/MS and MS3 together with accurate mass measurement of the molecular ions and of the collision induced dissociation (CID) fragments by liquid chromatography electro spray ionization time-of-flight mass spectrometry (LC/ESI-TOF).
如今，有必要识别制药行业中新型药物研发过程中出现的相关化合物。为此，除其他可用分析方法外，准确分子量的测定和经验式计算对于结构解析非常重要。在这项工作中，将展示在应激条件下获得的抗生素药物阿莫西林成品剂型中降解产物的鉴定和确认。利用液相色谱（LC）离子阱串联质谱（MS/MS）和三级质谱（MS3），结合液相色谱电喷雾电离飞行时间质谱（LC/ESI-TOF）对分子离子和碰撞诱导解离（CID）碎片进行精确质量测量来进行结构解析。
