Ecke Alexander, Schneider Rudolf J
BAM Federal Institute for Materials Research and Testing, 12205 Berlin, Germany.
Department of Chemistry, Humboldt-Universität zu Berlin, 12489 Berlin, Germany.
Antibiotics (Basel). 2021 Mar 12;10(3):298. doi: 10.3390/antibiotics10030298.
Contamination of waters with pharmaceuticals is an alarming problem as it may support the evolution of antimicrobial resistance. Therefore, fast and cost-effective analytical methods for potential on-site analysis are desired in order to control the water quality and assure the safety of its use as a source of drinking water. Antibody-based methods, such as the enzyme-linked immunosorbent assay (ELISA), can be helpful in this regard but can also have certain pitfalls in store, depending on the analyte. As shown here for the class of β-lactam antibiotics, hydrolysis of the β-lactam ring is a key factor in the immunochemical analysis as it influences antibody recognition. With the antibody used in this study, the limit of detection (LOD) in the immunoassay could be significantly reduced by hydrolysis for the five tested penicillins, with the lowest LOD for carbenicillin (0.2 nmol/L) and the greatest impact on penicillins G and V (reduction by 85%). In addition to enhanced quantification, our strategy also provides access to information about the degree of hydrolysis in water samples as shown for the most abundant penicillin amoxicillin.
水体受到药物污染是一个令人担忧的问题，因为它可能助长抗菌药物耐药性的演变。因此，需要快速且经济高效的分析方法用于潜在的现场分析，以控制水质并确保其作为饮用水源使用的安全性。基于抗体的方法，如酶联免疫吸附测定（ELISA），在这方面可能会有所帮助，但根据分析物的不同也可能存在某些隐患。如此处针对β-内酰胺类抗生素所示，β-内酰胺环的水解是免疫化学分析中的一个关键因素，因为它会影响抗体识别。对于本研究中使用的抗体，通过水解可显著降低免疫测定中五种受试青霉素的检测限（LOD），羧苄青霉素的最低检测限为0.2 nmol/L，对青霉素G和V的影响最大（降低85%）。除了提高定量分析能力外，我们的策略还能获取水样中水解程度的信息，如含量最丰富的青霉素阿莫西林所示。
