Kaneko Makoto, Takafuta Toshiro, Cuyun-Lira Olga, Satoh Kaneo, Arai Morio, Yatomi Yutaka, Ozaki Yukio
Department of Clinical and Laboratory Medicine, Faculty of Medicine, University of Yamanashi, Shimokato, Tamaho, Nakakoma, Yamanashi, Japan.
J Lab Clin Med. 2005 Aug;146(2):64-75. doi: 10.1016/j.lab.2005.04.007.
We previously reported that platelet retention rates as measured with collagen-coated bead columns (the conventional column) reflect the processes of platelet adhesion and aggregation under low shear stress, and that this system could serve as an easy-to-use platelet aggregometry. With this column, platelet glycoprotein (GP) VI and GPIIb/IIIa, but not the GPIb-von Willebrand factor (VWF) interaction, play major roles in platelet activation. To develop a system that can better reflect the GPIb-VWF interaction under high shear stress, we designed a column containing small-sized beads (125-212 microm) coated with porcine collagen type I. As expected, the GPIb-VWF interaction played a crucial role in platelet retention rates at higher flow rates. Adenosine 5'-diphosphate, but not thromboxane A2, appears to support platelet activation in this system. The platelet retention rates among healthy individuals with the new columns are in the range wider than the conventional columns, and this diversity could be attributed to the broad range of the VWF antigen and/or its activity. It is suggested that this new column can serve as an easy-to-use method for evaluating the VWF antigen levels and its activity and for monitoring patients with thrombotic or bleeding disorders related to the VWF-GPIb interaction.
我们之前报道过，用胶原包被的珠柱（传统柱）测量的血小板滞留率反映了低剪切应力下血小板黏附和聚集的过程，并且该系统可作为一种易于使用的血小板聚集测定法。使用此柱时，血小板糖蛋白（GP）VI和GPIIb/IIIa而非GPIb-血管性血友病因子（VWF）相互作用在血小板激活中起主要作用。为了开发一种能够更好地反映高剪切应力下GPIb-VWF相互作用的系统，我们设计了一种柱，其中含有包被有I型猪胶原的小珠子（125 - 212微米）。正如预期的那样，GPIb-VWF相互作用在较高流速下的血小板滞留率中起关键作用。在该系统中，5'-二磷酸腺苷而非血栓素A2似乎支持血小板激活。新柱在健康个体中的血小板滞留率范围比传统柱更宽，这种差异可能归因于VWF抗原及其活性的广泛范围。提示这种新柱可作为一种易于使用的方法，用于评估VWF抗原水平及其活性，以及监测与VWF-GPIb相互作用相关的血栓形成或出血性疾病患者。
