Moreira Nathalie, Mendes Filipa, Hogg Tim, Vasconcelos Isabel
Escola Superior de Biotecnologia, Universidade Católica Portuguesa, Rua Dr. António Bernardino de Almeida, 4200-072 Porto, Portugal.
Int J Food Microbiol. 2005 Sep 15;103(3):285-94. doi: 10.1016/j.ijfoodmicro.2004.12.029.
Strains of Hanseniaspora uvarum, Hanseniaspora guilliermondii and Saccharomyces cerevisiae were used as pure or mixed starter cultures in commercial medium, in order to compare their kinetic parameters and fermentation patterns. In pure and mixed cultures, yeasts presented similar ethanol yield and productivity. Pure cultures of H. uvarum and S. cerevisiae showed a specific growth rate of 0.38 h(-1); however, this value decreased when these yeasts were grown in mixed cultures with H. guilliermondii. The specific growth rate of pure cultures of H. guilliermondii was 0.41 h(-1) and was not affected by growth of other yeasts. H. guilliermondii was found to be the best producer of 2-phenylethyl acetate and 2-phenylethanol in both pure and mixed cultures. In pure cultures, H. uvarum led to the highest contents of heavy sulphur compounds, but H. guilliermondii and S. cerevisiae produced similar levels of methionol and 2-methyltetrahydrothiophen-3-one. Growth of apiculate yeasts in mixed cultures with S. cerevisiae led to amounts of 3-methylthiopropionic acid, acetic acid-3-(methylthio)propyl ester and 2-methyltetrahydrothiophen-3-one similar to those obtained in a pure culture of S. cerevisiae; however, growth of apiculate yeasts increased methionol contents of fermented media.
将葡萄汁有孢汉逊酵母、季也蒙有孢汉逊酵母和酿酒酵母菌株作为纯培养物或混合发酵剂，接种于商业培养基中，以比较它们的动力学参数和发酵模式。在纯培养和混合培养中，酵母的乙醇产量和生产率相似。葡萄汁有孢汉逊酵母和酿酒酵母的纯培养物的比生长速率为0.38 h⁻¹；然而，当这些酵母与季也蒙有孢汉逊酵母混合培养时，该值会降低。季也蒙有孢汉逊酵母纯培养物的比生长速率为0.41 h⁻¹，不受其他酵母生长的影响。发现在纯培养和混合培养中，季也蒙有孢汉逊酵母都是乙酸苯乙酯和苯乙醇的最佳生产者。在纯培养中，葡萄汁有孢汉逊酵母产生的重硫化合物含量最高，但季也蒙有孢汉逊酵母和酿酒酵母产生的甲醇和2-甲基四氢噻吩-3-酮水平相似。与酿酒酵母混合培养时，尖状酵母的生长导致3-甲基硫代丙酸、3-（甲硫基）丙基乙酸酯和2-甲基四氢噻吩-3-酮的含量与酿酒酵母纯培养时相似；然而，尖状酵母的生长增加了发酵培养基中甲醇的含量。
