从芦竹根茎中分离出具有抗增殖活性的N-乙酰-D-葡萄糖胺特异性凝集素。

Isolation of an N-acetyl-D-glucosamine specific lectin from the rhizomes of Arundo donax with antiproliferative activity.

作者信息

Kaur Amandeep, Singh Jatinder, Kamboj Sukhdev Singh, Sexana A K, Pandita Renu Moti, Shamnugavel M

机构信息

Department of Molecular Biology & Biochemistry, Guru Nanak Dev University, Amritsar 143 005, Punjab, India.

出版信息

Phytochemistry. 2005 Aug;66(16):1933-40. doi: 10.1016/j.phytochem.2005.06.026.

DOI:10.1016/j.phytochem.2005.06.026

PMID:16099485

Abstract

A lectin with antiproliferative activity towards human cancer cell lines and mitogenic towards human peripheral blood mononuclear cells was purified from the rhizomes of Arundo donax (Linn.) by affinity chromatography on N-acetyl-d-glucosamine linked to epoxy-activated sepharose-6B. The pure preparation apparently yielded a single band of approximately 15 kDa on SDS-PAGE, pH 8.3, under both reducing and non-reducing conditions. The molecular mass of native lectin was 32 kDa as determined by gel filtration chromatography. This showed the lectin to be a dimer, with subunits not held together by disulphide linkages. The A. donax lectin (ADL) agglutinated rabbit erythrocytes and the agglutination was inhibited by N-acetyl-d-glucosamine and its di- and trimer. The lectin was thermostable upto 55 degrees C and showed optimum activity in the range of pH 7.0-9.0 and comprised of 2.1% carbohydrate content.

摘要

从芦竹（Arundo donax (Linn.)）的根茎中，通过在与环氧活化琼脂糖-6B相连的N-乙酰-d-葡萄糖胺上进行亲和层析，纯化出一种对人类癌细胞系具有抗增殖活性且对人外周血单个核细胞具有促有丝分裂活性的凝集素。在SDS-PAGE（pH 8.3）中，无论是在还原条件还是非还原条件下，该纯制剂在约15 kDa处明显呈现出一条单一的条带。通过凝胶过滤色谱法测定，天然凝集素的分子量为32 kDa。这表明该凝集素是一种二聚体，其亚基并非通过二硫键连接在一起。芦竹凝集素（ADL）能凝集兔红细胞，且凝集作用被N-乙酰-d-葡萄糖胺及其二聚体和三聚体所抑制。该凝集素在高达55℃时具有热稳定性，在pH 7.0 - 9.0范围内表现出最佳活性，碳水化合物含量为2.1%。