Ameriso Sebastian F, Villamil Agustina Ruiz, Zedda Christine, Parodi Juan C, Garrido Sergio, Sarchi María Inés, Schultz Marcelo, Boczkowski Jorge, Sevlever Gustavo E
Institute for Neurological Research, FLENI, Buenos Aires, Argentina.
Stroke. 2005 Sep;36(9):1896-900. doi: 10.1161/01.STR.0000177494.43587.9e. Epub 2005 Aug 11.
It is not well established what are the features, if any, that distinguish symptomatic from asymptomatic carotid atherosclerotic plaques. Inducible heme oxygenase-1 (HO-1) is a component of cellular defense mechanisms against oxidative stress. We aimed to assess the presence of Helicobacter pylori (H pylori) and the expression of HO-1 in carotid atherosclerotic plaques of patients with and without prior neurologic symptoms attributable to the operated artery.
We examined 25 symptomatic and 23 asymptomatic carotid atherosclerotic plaques removed during endarterectomy and 7 normal carotid arteries obtained at autopsy. We investigated the presence of H pylori DNA in the vessel wall and performed immunohistochemical detection of HO-1.
H pylori DNA was present in 28 plaques and HO-1 was expressed in 30 plaques. HO-1 was found in 27 H pylori-positive specimens but in only 3 H pylori-negative specimens (P<0.001). All 7 normal carotid arteries were negative for both H pylori and HO-1. Although 82% of asymptomatic specimens were positive for H pylori and 87% for HO-1, only 36% of symptomatic specimens were positive for both H pylori and HO-1 (P<0.01).
This study suggests a strong association between H pylori infection and expression of HO-1 in carotid atherosclerotic plaques. There was a substantial prevalence of these features in specimens obtained from asymptomatic subjects.
目前尚不清楚有哪些特征（如果有的话）可区分有症状和无症状的颈动脉粥样硬化斑块。诱导型血红素加氧酶-1（HO-1）是细胞抗氧化应激防御机制的一个组成部分。我们旨在评估幽门螺杆菌（H pylori）的存在情况以及HO-1在有或无手术相关动脉所致既往神经症状的患者颈动脉粥样硬化斑块中的表达。
我们检查了25个在颈动脉内膜切除术中切除的有症状的颈动脉粥样硬化斑块、23个无症状的颈动脉粥样硬化斑块以及7个尸检获得的正常颈动脉。我们研究了血管壁中幽门螺杆菌DNA的存在情况，并对HO-1进行免疫组化检测。
28个斑块中存在幽门螺杆菌DNA，30个斑块中表达HO-1。在27个幽门螺杆菌阳性标本中发现了HO-1，但在仅3个幽门螺杆菌阴性标本中发现了HO-1（P<0.001）。所有7个正常颈动脉的幽门螺杆菌和HO-1均为阴性。虽然82%的无症状标本幽门螺杆菌阳性，87%的无症状标本HO-1阳性，但有症状标本中仅36%的幽门螺杆菌和HO-1均为阳性（P<0.01）。
本研究提示幽门螺杆菌感染与颈动脉粥样硬化斑块中HO-1的表达之间存在密切关联。在从无症状受试者获得的标本中，这些特征的发生率相当高。
