Yan Hai
Department of Pathology, Duke University Meeical Center, Durham, NC, USA.
Methods Mol Biol. 2005;311:31-8. doi: 10.1385/1-59259-957-5:031.
The analysis of gene differential expression is complicated by the potentially subtle differences associated with alterations in a single allele as well as by variations between individuals that arise from environmental or physiological factors. To circumvent these analytic problems, a method, named allele-specific differential expression analysis, was developed to compare the relative expression levels of two alleles of the same gene within the same cellular sample. The studies of allele-specific expression revealed that differential expression is relatively common in the human population.
基因差异表达的分析因与单个等位基因改变相关的潜在细微差异以及个体间由环境或生理因素引起的变异而变得复杂。为了规避这些分析问题，人们开发了一种名为等位基因特异性差异表达分析的方法，用于比较同一细胞样本中同一基因两个等位基因的相对表达水平。对等位基因特异性表达的研究表明，差异表达在人类群体中相对常见。
