Wjst Matthias, van den Boom Dirk
Molecular Epidemiology, GSF-Forschungszentrum für Umwelt und Gesundheit, Neuherberg/Munich, Germany.
Methods Mol Biol. 2005;311:125-37. doi: 10.1385/1-59259-957-5:125.
Major strengths of mass spectrometry analysis include the accuracy of the detection principle and the automatic data storage, making it a premier choice for high-throughput single-nucleotide polymorphism genotyping. We explain the assay principle in detail and give step-by-step laboratory instructions. Finally, references point toward further use of mass spectrometry analysis for molecular haplotyping, pooled DNA analysis, re-sequencing, and gene expression studies.
质谱分析的主要优势包括检测原理的准确性和数据的自动存储，这使其成为高通量单核苷酸多态性基因分型的首选方法。我们详细解释了该检测原理，并给出了实验室操作的详细步骤说明。最后，参考文献指出了质谱分析在分子单倍型分析、混合DNA分析、重测序和基因表达研究中的进一步应用。
