Sato Kazuhiro, Kusaka Yukinori, Suganuma Narufumi, Akino Hironobu, Yokoyama Osamu
Department of Environmental Health, School of Medicine, University of Fukui, Fukui 910-1193, Japan.
Ind Health. 2005 Jul;43(3):574-9. doi: 10.2486/indhealth.43.574.
Co and Ni are essential but relatively rare elements as to organisms. In the mammalian membrane, these metals are transported by the same carrier proteins. The aim of this study was to investigate the direct effects of CoCl2 and NiCl2 on citrate uptake by rat renal brush border membrane vesicles (BBMV). BBMV were prepared by the divalent cation precipitation methods, and citrate uptake was measured by the Millipore rapid membrane filtration technique. The time course of citrate uptake during 120-min of incubation with 1 mM CoCl2 and NiCl2 showed a rapid significant inhibition at the early phase and a slight recover at the late phase. Incubation for 1 min of BBMV with 1, 5 and 25 mM CoCl2 and NiCl2, respectively, significantly inhibited citrate uptake in a concentration-dependent manner compared with that of 0 mM. We discuss these findings from the point of view that Co and Ni are located in Group VIII of the periodic table.
钴和镍对生物体而言是必需但相对稀有的元素。在哺乳动物细胞膜中，这些金属由相同的载体蛋白运输。本研究的目的是调查氯化钴和氯化镍对大鼠肾刷状缘膜囊泡（BBMV）摄取柠檬酸盐的直接影响。通过二价阳离子沉淀法制备BBMV，并采用密理博快速膜过滤技术测量柠檬酸盐摄取量。在与1 mM氯化钴和氯化镍孵育120分钟期间，柠檬酸盐摄取的时间进程显示在早期有快速显著的抑制，在后期有轻微恢复。分别用1、5和25 mM氯化钴和氯化镍孵育BBMV 1分钟，与0 mM相比，显著抑制柠檬酸盐摄取，呈浓度依赖性。我们从钴和镍位于元素周期表第八族的角度讨论这些发现。
