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A method for cytogenetic analysis of boar spermatozoa using hamster oocytes.

作者信息

Benkhalifa M, Bonneau M, Popescu P, Boscher J, Boucher D, Malet P

机构信息

Laboratoire de Cytogénétique Médicale, Faculté de Médecine, Clermont-Ferrand, France.

出版信息

Ann Genet. 1992;35(1):61-4.

PMID:1610124
Abstract

In this paper, the authors detail a method for displaying boar spermatozoa chromosomes using heterospecific zona-free hamster oocyte penetration technique. Semen samples from two Large-White boars having a normal spermogram were studied. The first one had a normal karyotype (38,XY), the second carried a reciprocal translocation rcp(3;7)(p1,3;q2,1). After in vitro fertilization by capacitated sperm, culture and cytogenetic analysis of hamster eggs we obtained metaphase spreads of spermatozoa chromosomes. The ratio of X- and Y-bearing spermatozoa was 49.2% and 50.8%, respectively.

摘要

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