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Screening and purification of a novel trypsin inhibitor from Prosopis juliflora seeds with activity toward pest digestive enzymes.

作者信息

Sivakumar S, Franco O L, Tagliari P D, Bloch C, Mohan M, Thayumanavan B

机构信息

Center for Plant Molecular Biology, Tamil Nadu and Agricultural University, Coimbatore-641003, India.

出版信息

Protein Pept Lett. 2005 Aug;12(6):561-5. doi: 10.2174/0929866054395897.

DOI:10.2174/0929866054395897
PMID:16101396
Abstract

Several pests are capable of decreasing crop production causing severe economical and social losses. Aiming to find novel molecules that could impede the digestion process of different pests, a screening of alpha-amylase and trypsin-like proteinase inhibitors was carried out in Prosopis juliflora, showing the presence of both in dry seeds. Furthermore, a novel trypsin inhibitor, with molecular mass of 13,292 Da, was purified showing remarkable in vitro activity against T. castaneum and C. maculatus.

摘要

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