创新的多功能序列寡肽载体Soc(n)-I和SOC(n)-II：功能-技术-展望

Innovative, multifunctional sequential oligopeptide carriers Soc(n)-I and SOC(n)-II: functions-technology-perspectives.

作者信息

Alexopoulos Charalampos, Krikorian Dimitrios, Panou-Pomonis Eugenia, Sakarellos-Daitsiotis Maria, Sakarellos Constantinos

机构信息

Laboratory of Peptide Chemistry, University of Ioannina, 45 110 Ioannina, Greece.

出版信息

Protein Pept Lett. 2005 Aug;12(6):601-7. doi: 10.2174/0929866054395914.

DOI:10.2174/0929866054395914

PMID:16101403

Abstract

An innovative type of multifunctional helicoid artificial carriers, formed by the repetitive Lys-Aib-Gly (SOC(n)-I) or by the Aib-Lys-Aib-Gly (SOC(n)-II), with structural rigidity and regularity were successfully applied in our laboratory for anchoring antigenic/immunogenic peptides. The carriers, designed to display a predetermined 3D structure, adopt the 3(10) helical conformation, while the attached peptides preserve their original "active" conformation. The constructed conjugates were used as substrates in solid phase immunoassays, as well as for generating potent and specific immune responses.

摘要

一种由重复的Lys-Aib-Gly（SOC(n)-I）或Aib-Lys-Aib-Gly（SOC(n)-II）形成的具有结构刚性和规则性的创新型多功能螺旋体人工载体，已在我们实验室成功用于锚定抗原性/免疫原性肽。这些载体设计为呈现预定的三维结构，采用3(10)螺旋构象，而附着的肽保留其原始的“活性”构象。构建的缀合物用作固相免疫测定的底物，也用于产生强效且特异性的免疫反应。