Radziwon P, Boczkowska-Radziwon B, Lipska A, Smoktunowicz M, Kloczko J
Regional Centre for Transfusion Medicine, Bialystok, Poland.
Transfus Med. 2005 Aug;15(4):319-22. doi: 10.1111/j.0958-7578.2005.00594.x.
Blood circulating in extracorporeal circuit of the apheresis sets has a contact with an artificial surface. The data on the influence of plateletpheresis on fibrinolytic activity are very limited and difficult to interpret. The aim of our study was to estimate the effect of plateletpheresis on the activation of fibrinolysis. Plateletpheresis was performed in 17 healthy blood donors using continuous-flow cell separator COM.TEC (Fresenius, Bad Homburg, Germany). Before and after plateletpheresis, blood samples were taken and markers of fibrinolysis (PAP, t-PA, PAI-1) as well as factor XII activity have been measured. We observed statistically significant decrease in t-PA and factor XII activities after plateletpheresis. There were no significant changes in concentrations of t-PA, PAI-1 and PAP as well as PAI-1 activity after plateletpheresis. Plateletpheresis performed by COM.TEC cell separator has very little, if any, effect on the activation of fibrinolysis. The mechanism of the inhibition of t-PA activity needs further investigations.
血液单采设备体外循环回路中的循环血液会与人工表面接触。关于血小板单采对纤溶活性影响的数据非常有限且难以解读。我们研究的目的是评估血小板单采对纤溶激活的影响。使用连续流动细胞分离器COM.TEC（费森尤斯公司，德国巴特洪堡）对17名健康献血者进行血小板单采。在血小板单采前后采集血样，并检测纤溶标志物（纤溶酶原激活物抑制物-1、组织型纤溶酶原激活物、纤溶酶-抗纤溶酶复合物）以及因子Ⅻ活性。我们观察到血小板单采后组织型纤溶酶原激活物和因子Ⅻ活性有统计学意义的降低。血小板单采后组织型纤溶酶原激活物、纤溶酶原激活物抑制物-1和纤溶酶-抗纤溶酶复合物的浓度以及纤溶酶原激活物抑制物-1活性均无显著变化。使用COM.TEC细胞分离器进行血小板单采对纤溶激活的影响极小（如果有影响的话）。组织型纤溶酶原激活物活性受抑制的机制需要进一步研究。
