Gibson K J, Gibson J
Central Research and Development Department, E. I. du Pont de Nemours and Company, Wilmington, Delaware 19880-0402.
Appl Environ Microbiol. 1992 Feb;58(2):696-8. doi: 10.1128/aem.58.2.696-698.1992.
Alkali-treated extracts of Rhodopseudomonas palustris growing photosynthetically on benzoate were examined by gas chromatography/mass spectrometry for partially reduced benzoate derivatives. Two cyclic dienes, cyclohexa-2,5-diene-1-carboxylate and cyclohexa-1,4-diene-1-carboxylate, were detected. Either compound supported cell growth as effectively as benzoate. These results suggest that these cyclohexadienecarboxylates, probably as their coenzyme A esters, are the initial reduction products formed during anaerobic benzoate metabolism by R. palustris.
利用气相色谱/质谱联用技术对在苯甲酸上进行光合生长的沼泽红假单胞菌经碱处理后的提取物进行检测,以寻找部分还原的苯甲酸衍生物。检测到两种环状二烯,即环己-2,5-二烯-1-羧酸酯和环己-1,4-二烯-1-羧酸酯。这两种化合物中的任何一种都能像苯甲酸一样有效地支持细胞生长。这些结果表明,这些环己二烯羧酸酯,可能是以其辅酶A酯的形式,是沼泽红假单胞菌在厌氧苯甲酸代谢过程中形成的初始还原产物。