环核苷酸磷酸二酯酶3A在分离的大鼠下颌下腺腺泡中的表达

Expression of cyclic nucleotide phosphodiesterase 3A in isolated rat submandibular acini.

作者信息

Shimizu Kasumi, Murata Taku, Hiramoto Kenichi, Sugiyama Takeo, Nakagawa Toshiyuki, Manganiello Vincent C, Tagawa Toshiro

机构信息

Department of Oral and Maxillofacial Surgery, Faculty of Medicine, Mie University, 2-174 Edobashi, Tsu, Mie 514-8507, Japan.

出版信息

Arch Oral Biol. 2006 Feb;51(2):83-8. doi: 10.1016/j.archoralbio.2005.06.012. Epub 2005 Aug 15.

DOI:10.1016/j.archoralbio.2005.06.012

PMID:16102722

Abstract

Phosphodiesterase (PDE) 3 has been characterized in isolated rat submandibular acini. PDE3 activity was detected in homogenates of isolated rat submandibular acini; little or no PDE3 activity was found in ducts. About 62% of PDE3 activity in the acini was recovered in the supernatant fractions; 38% in particulate fractions. In the acini, but not ducts, PDE3A mRNA was detected by reverse transcriptase-polymerase chain reaction (RT-PCR). The PDE3-specific inhibitor, cilostamide, increased the ratio of apomucin mRNA/18s rRNA, as quantified by real-time RT-PCR. Our results indicate that PDE3A may be important in regulating cAMP pools that control acini functions.

摘要

磷酸二酯酶（PDE）3已在分离的大鼠下颌下腺腺泡中得到表征。在分离的大鼠下颌下腺腺泡匀浆中检测到了PDE3活性；在导管中几乎未检测到或未检测到PDE3活性。腺泡中约62%的PDE3活性存在于上清液部分；38%存在于颗粒部分。通过逆转录聚合酶链反应（RT-PCR）在腺泡而非导管中检测到了PDE3A mRNA。磷酸二酯酶3特异性抑制剂西洛酰胺增加了通过实时RT-PCR定量的脱辅基粘蛋白mRNA/18s核糖体RNA的比率。我们的结果表明，PDE3A可能在调节控制腺泡功能的环磷酸腺苷（cAMP）池方面发挥重要作用。