银大麻哈鱼（Oncorhynchus kisutch）性染色体对的鉴定：与大鳞大麻哈鱼（Oncorhynchus tshawytscha）性连锁群缺乏保守性。
Identification of the sex chromosome pair in coho salmon (Oncorhynchus kisutch): lack of conservation of the sex linkage group with chinook salmon (Oncorhynchus tshawytscha).
作者信息
Phillips R B, Morasch M R, Park L K, Naish K A, Devlin R H
机构信息
Department of Biological Sciences, Washington State University, Vancouver, WA, USA.
出版信息
Cytogenet Genome Res. 2005;111(2):166-70. doi: 10.1159/000086387.
Fluorescence in situ hybridization (FISH) using a probe to the male-specific GH-Y (growth hormone pseudogene) was used to identify the Y chromosome in coho salmon (Oncorhynchus kisutch). The sex chromosome pair is morphologically similar to chinook salmon (Oncorhynchus tshawytscha) with the GH-Y localized to the small short arm of the largest subtelocentric chromosome pair. FISH experiments with probes containing sex-linked genes in rainbow trout (Oncorhynchus mykiss) (SCAR163) and chinook salmon (Omy7INRA) showed that the coho sex linkage group is different from chinook and rainbow trout and this was confirmed by segregation analysis for the Omy7INRA locus. The telomeric location of the SEX locus, the presence of shared male-specific markers in coho and chinook salmon, and the lack of conservation of sex-linkage groups suggest that transposition of a small male-specific region may have occurred repeatedly in salmonid fishes of the genus Oncorhynchus.
使用针对雄性特异性生长激素假基因（GH-Y）的探针进行荧光原位杂交（FISH），以鉴定银大麻哈鱼（Oncorhynchus kisutch）中的Y染色体。性染色体对在形态上与大鳞大麻哈鱼（Oncorhynchus tshawytscha）相似，GH-Y定位于最大的亚端着丝粒染色体对的小短臂上。用含有虹鳟（Oncorhynchus mykiss）（SCAR163）和大鳞大麻哈鱼（Omy7INRA）性连锁基因的探针进行的FISH实验表明，银大麻哈鱼的性连锁群与大鳞大麻哈鱼和虹鳟不同，这通过对Omy7INRA位点的分离分析得到了证实。SEX位点的端粒位置、银大麻哈鱼和大鳞大麻哈鱼中共享的雄性特异性标记的存在，以及性连锁群缺乏保守性，表明一个小的雄性特异性区域的转座可能在大麻哈鱼属的鲑科鱼类中反复发生。
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