大麻哈鱼（Oncorhynchus keta）和细鳞大麻哈鱼（Oncorhynchus gorbuscha）性染色体对的鉴定。

Identification of the sex chromosome pair in chum salmon (Oncorhynchus keta) and pink salmon (Oncorhynchus gorbuscha).

作者信息

Phillips R B, DeKoning J, Morasch M R, Park L K, Devlin R H

机构信息

Department of Biological Sciences, Washington State University, Vancouver, WA 98686-9600, USA.

出版信息

Cytogenet Genome Res. 2007;116(4):298-304. doi: 10.1159/000100414.

DOI:10.1159/000100414

PMID:17431328

Abstract

Fluorescence in situ hybridization (FISH) using a probe to the male-specific GH-Y (growth hormone pseudogene) was used to identify the Y chromosome in the karyotypes of chum salmon (Oncorhynchus keta) and pink salmon (Oncorhynchus gorbuscha). The sex chromosome pair is a small acrocentric chromosome pair in chum salmon and the smallest metacentric chromosome pair in pink salmon. Both of these chromosome pairs are morphologically different from the sex chromosome pairs in chinook salmon (Oncorhynchus tshawytscha) and coho salmon (Oncorhynchus kisutch). The 5S rRNA genes are on multiple chromosome pairs including the sex chromosome pair in chum salmon, but at the centromeres of two autosomal metacentric pairs in pink salmon. The sex chromosome pairs and the chromosomal locations of the 5S rDNA appear to be different in all five of the North American Pacific salmon species and rainbow trout. The implications of these results for evolution of sex chromosomes in salmonids are discussed.

摘要

使用针对雄性特异性生长激素假基因（GH-Y）的探针进行荧光原位杂交（FISH），以鉴定马苏大麻哈鱼（Oncorhynchus keta）和驼背大麻哈鱼（Oncorhynchus gorbuscha）核型中的Y染色体。性染色体对在马苏大麻哈鱼中是一对小的近端着丝粒染色体，在驼背大麻哈鱼中是最小的中着丝粒染色体对。这两对染色体在形态上均与大鳞大麻哈鱼（Oncorhynchus tshawytscha）和银大麻哈鱼（Oncorhynchus kisutch）的性染色体对不同。5S rRNA基因存在于多对染色体上，包括马苏大麻哈鱼的性染色体对，但在驼背大麻哈鱼中位于两对常染色体中着丝粒处。在所有五种北美太平洋鲑鱼和虹鳟中，性染色体对以及5S rDNA的染色体位置似乎都不同。本文讨论了这些结果对鲑科鱼类性染色体进化的影响。