钙调蛋白特异性结合肌营养不良蛋白 - 糖蛋白复合物的三种蛋白质。

Calmodulin specifically binds three proteins of the dystrophin-glycoprotein complex.

作者信息

Madhavan R, Massom L R, Jarrett H W

机构信息

Department of Biochemistry, University of Tennessee, Memphis 38163.

出版信息

Biochem Biophys Res Commun. 1992 Jun 15;185(2):753-9. doi: 10.1016/0006-291x(92)91690-r.

DOI:10.1016/0006-291x(92)91690-r

PMID:1610366

Abstract

Dystrophin is the approximately 400,000 Da. protein (p400K) product of the Duchenne muscular dystrophy gene locus. In the sarcolemma membrane, it is associated with several other proteins, many of which are glycoproteins (abbreviated gp) and include gp156K, p59K, gp50K, gp43K, gp35K, and p25K. Here, we show that dystrophin, gp156K, and p59K are calmodulin-binding proteins, the binding is Ca(2+)-dependent, and of high-affinity similar to that seen with calmodulin-activated enzymes. Two putative calmodulin-binding sequences were identified, one at either end of the dystrophin sequence.

摘要

肌营养不良蛋白是杜氏肌营养不良基因位点产生的约400,000道尔顿的蛋白质（p400K）。在肌膜中，它与其他几种蛋白质相关联，其中许多是糖蛋白（缩写为gp），包括gp156K、p59K、gp50K、gp43K、gp35K和p25K。在此，我们表明肌营养不良蛋白、gp156K和p59K是钙调蛋白结合蛋白，这种结合是Ca(2+)依赖性的，并且具有与钙调蛋白激活酶相似的高亲和力。鉴定出两个推定的钙调蛋白结合序列，一个位于肌营养不良蛋白序列的一端，另一个位于另一端。