Thomsen L L, Ching L M, Joseph W R, Baguley B C, Gavin J B
Cancer Research Laboratory, University of Auckland School of Medicine, New Zealand.
Biochem Pharmacol. 1992 Jun 9;43(11):2401-6. doi: 10.1016/0006-2952(92)90319-e.
The production of nitric oxide in endotoxin-resistant C3H/HeJ mice in response to flavone-8-acetic acid (FAA), derivatives of xanthenone-4-acetic (XAA), endotoxin and recombinant human tumour necrosis factor-alpha (TNF-alpha) was investigated and compared with the induction of haemorrhagic necrosis in subcutaneous M16/C tumours. FAA and XAA analogues stimulated nitric oxide production both in vitro (activated macrophages) and in vivo (plasma nitrate elevation) in both C3H/HeJ and C3H/HeN mice (5,6-dimethyl-XAA greater than 5-methyl-XAA greater than FAA greater than XAA greater than 8-methyl-XAA). Recombinant human TNF-alpha stimulated nitric oxide production equally from both murine strains while endotoxin stimulated nitric oxide production only by C3H/HeN mice. The extent of induction of haemorrhagic necrosis in tumour-bearing mice treated with FAA, 5,6-dimethyl XAA or endotoxin paralleled the effects on nitric oxide production, showing a differential between the two strains of mice only in the case of endotoxin.
研究了内毒素抗性C3H/HeJ小鼠对黄酮 - 8 - 乙酸（FAA）、呫吨酮 - 4 - 乙酸（XAA）衍生物、内毒素和重组人肿瘤坏死因子 - α（TNF - α）的一氧化氮产生情况，并与皮下M16/C肿瘤出血性坏死的诱导情况进行了比较。FAA和XAA类似物在体外（活化巨噬细胞）和体内（血浆硝酸盐升高）均刺激C3H/HeJ和C3H/HeN小鼠产生一氧化氮（5,6 - 二甲基 - XAA＞5 - 甲基 - XAA＞FAA＞XAA＞8 - 甲基 - XAA）。重组人TNF - α对两种小鼠品系产生一氧化氮的刺激作用相同，而内毒素仅刺激C3H/HeN小鼠产生一氧化氮。用FAA、5,6 - 二甲基XAA或内毒素处理的荷瘤小鼠的出血性坏死诱导程度与对一氧化氮产生的影响平行，仅在内毒素的情况下两种小鼠品系之间存在差异。
