核糖2'-F标记：一种通过19F核磁共振光谱表征RNA二级结构平衡的简单工具。
Ribose 2'-F labeling: a simple tool for the characterization of RNA secondary structure equilibria by 19F NMR spectroscopy.
作者信息
Kreutz Christoph, Kählig Hanspeter, Konrat Robert, Micura Ronald
机构信息
Institute of Organic Chemistry, Center for Molecular Biosciences CMBI, University of Innsbruck, Innsbruck, Austria.
出版信息
J Am Chem Soc. 2005 Aug 24;127(33):11558-9. doi: 10.1021/ja052844u.
We present a facile 19F NMR spectroscopic approach for the characterization of bistable RNA. The concept is based on the site-specific labeling of RNA with single 2'-deoxy-2'-fluoro (2'-F) nucleosides. Depending on the position of the 2'-F label (either within a double helix or a single-stranded region), significantly different 19F chemical shifts were observed and assigned to the particular alternative folds. This has been exemplified for a series of bistable RNAs. The approach is promising for the detailed investigation of complex RNA folding pathways.
我们提出了一种用于表征双稳态RNA的简便的19F核磁共振光谱方法。该概念基于用单个2'-脱氧-2'-氟（2'-F）核苷对RNA进行位点特异性标记。根据2'-F标记的位置（在双螺旋内或单链区域内），观察到明显不同的19F化学位移，并将其指定为特定的替代折叠形式。这已通过一系列双稳态RNA得到了例证。该方法对于详细研究复杂的RNA折叠途径很有前景。
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