Kreutz Christoph, Kählig Hanspeter, Konrat Robert, Micura Ronald
Institute of Organic Chemistry, Center for Molecular Biosciences CMBI, University of Innsbruck, Innsbruck, Austria.
J Am Chem Soc. 2005 Aug 24;127(33):11558-9. doi: 10.1021/ja052844u.
We present a facile 19F NMR spectroscopic approach for the characterization of bistable RNA. The concept is based on the site-specific labeling of RNA with single 2'-deoxy-2'-fluoro (2'-F) nucleosides. Depending on the position of the 2'-F label (either within a double helix or a single-stranded region), significantly different 19F chemical shifts were observed and assigned to the particular alternative folds. This has been exemplified for a series of bistable RNAs. The approach is promising for the detailed investigation of complex RNA folding pathways.
我们提出了一种用于表征双稳态RNA的简便的19F核磁共振光谱方法。该概念基于用单个2'-脱氧-2'-氟（2'-F）核苷对RNA进行位点特异性标记。根据2'-F标记的位置（在双螺旋内或单链区域内），观察到明显不同的19F化学位移，并将其指定为特定的替代折叠形式。这已通过一系列双稳态RNA得到了例证。该方法对于详细研究复杂的RNA折叠途径很有前景。
