Gilmour Gary, Iversen Susan D, O'Neill Michael F, O'Neill Michael J, Ward Mark A, Bannerman David M
Eli Lilly and Co. Ltd., Lilly Research Centre, Erl Wood Manor, Sunninghill Road, Windlesham, Surrey GU20 6PH, UK.
Behav Brain Res. 2005 Nov 30;165(1):98-109. doi: 10.1016/j.bbr.2005.06.027. Epub 2005 Aug 18.
This study investigated the effect of amphetamine (AMP) on skilled forelimb use following focal cortical ischaemic lesions in the rat. Unilateral lesions were produced by a novel method of intracortical microinjection of endothelin-1 (ET-1), intended to principally target the forelimb representation zone in primary motor-primary somatosensory cortex. Lesions were placed in the hemisphere contralateral to the preferred limb and produced deficits in skilled forelimb use on two tasks: the paw reach (PR) test and the foot fault (FF) test. Beginning on post-lesion day (D) 2, animals received injections of 2 mg/kg AMP and were injected every third day until D26. Animals were tested both during, and 24 h after, AMP administration. AMP facilitated recovery of skilled forelimb use on the PR test when assessed during drug-free test sessions. No such effect was seen on the FF test. These results demonstrate that sub-acute administration of AMP following a unilateral focal ischaemic lesion of FL can facilitate task-dependent recovery of skilled forelimb use in the rat. They also demonstrate that different behavioural tasks measuring superficially similar behavioural outputs may show different sensitivities to such drug effects.
本研究调查了苯丙胺（AMP）对大鼠局灶性皮质缺血性损伤后熟练前肢使用能力的影响。采用一种新的脑内微注射内皮素-1（ET-1）的方法制造单侧损伤，主要针对初级运动-初级体感皮层中的前肢代表区。损伤位于偏好肢体对侧的半球，在两项任务中导致熟练前肢使用能力出现缺陷：爪够物（PR）试验和足部失误（FF）试验。从损伤后第2天开始，动物接受2mg/kg AMP注射，每三天注射一次，直至第26天。在AMP给药期间和给药后24小时对动物进行测试。在无药物测试期间评估时，AMP促进了PR试验中熟练前肢使用能力的恢复。在FF试验中未观察到这种效果。这些结果表明，在FL单侧局灶性缺血性损伤后亚急性给予AMP可促进大鼠熟练前肢使用能力的任务依赖性恢复。它们还表明，测量表面上相似行为输出的不同行为任务可能对这种药物效应表现出不同的敏感性。
