Bala Manju, Ray Krishna, Gupta S M
Regional STD Teaching, Training & Research Centre, Vardhman Mahavir Medical College & Safdarjang Hospital, New Delhi, India.
Indian J Med Res. 2005 Jul;122(1):48-51.
BACKGROUND & OBJECTIVE: objectives: As antimicrobial susceptibility testing of Neisseria gonorrhoeae provides guidance for appropriate treatment, there is a need for simple, reliable and cost-effective method for susceptibility testing. The present study was aimed to compare the results of two methods of susceptibility testing, minimum inhibitory concentration (MIC) values by E test with disc diffusion results by Australian Gonococcal Surveillance Programme (AGSP) method in N. gonorrhoeae isolates.
Susceptibility testing for ciprofloxacin, penicillin and ceftriaxone using AGSP method was carried out for 301 confirmed consecutive isolates of N. gonorrhoeae. MIC of ciprofloxacin, penicillin and ceftriaxone was determined by E test in 301, 198 and 128 isolates respectively. The results of the two methods were compared by using Kappa statistics.
Moderate levels of agreement for ciprofloxacin (kappa=0.44) and penicillin (kappa=0.54) were observed between the two methods. For ceftriaxone, 96.1 and 0.8 per cent isolates were found to be susceptible and less sensitive respectively by both the methods and per cent agreement between the two methods was 96.9 per cent.
INTERPRETATION & CONCLUSION: Both the methods were easy to perform and gave reproducible results. However, disc diffusion method was cost-effective and more feasible in routine diagnostic laboratories in developing countries like India.
淋病奈瑟菌的抗菌药物敏感性试验可为恰当治疗提供指导，因此需要一种简单、可靠且经济高效的药敏试验方法。本研究旨在比较两种药敏试验方法的结果，即采用E试验测定淋病奈瑟菌分离株的最低抑菌浓度（MIC）值，并与澳大利亚淋球菌监测计划（AGSP）方法的纸片扩散法结果进行比较。
采用AGSP方法对301株连续确诊的淋病奈瑟菌分离株进行环丙沙星、青霉素和头孢曲松的药敏试验。分别对301株、198株和128株分离株采用E试验测定环丙沙星、青霉素和头孢曲松的MIC。采用Kappa统计量比较两种方法的结果。
两种方法对环丙沙星（kappa = 0.44）和青霉素（kappa = 0.54）的一致性水平中等。对于头孢曲松，两种方法均发现96.1%的分离株敏感，0.8%的分离株敏感性较低，两种方法之间的一致率为96.9%。
两种方法操作简便且结果可重复。然而，纸片扩散法在印度等发展中国家的常规诊断实验室中具有成本效益且更可行。
