Bhalla P, Vidhani S, Reddy B S N, Chowdhry S, Mathur M D
Departments of Microbiology & Skin & STD, Maulana Azad Medical College & Associated, Lok Nayak Hospital, New Delhi, India.
Indian J Med Res. 2002 Mar;115:113-7.
BACKGROUND & OBJECTIVES: Treatment for gonorrhoea with fluoroquinolones is recommended. However, reduced susceptibility and treatment failure with fluoroquinolones has recently been reported. We undertook to study the antibiotic susceptibility pattern and the incidence of quinolone resistance in 36 consecutive isolates of Neisseria gonorrhoeae from April to November 2000.
Antibiotic susceptibility testing was performed by the Kirby Bauer disc diffusion technique and minimum inhibitory concentration (MIC) of ciprofloxacin was determined by the agar dilution method. Penicillinase producing N. gonorrhoeae (PPNG) were identified by using the nitrocefin disc method.
Thirty six strains of N. gonorrhoeae obtained from 44 consecutive male patients (81.9%) were studied. By the disc diffusion method, only 3 (8.3%) of these isolates were found to be sensitive to ciprofloxacin. All isolates were sensitive to ceftriaxone while 23 (63.9%) were sensitive to tetracycline and 12 (33.3%) to penicillin. Four (11.1%) of the N. gonorrhoeae isolates were PPNG. Twenty seven (75%) isolates were found to be resistant to ciprofloxacin by MIC determination.
INTERPRETATION & CONCLUSION: Incidence of ciprofloxacin resistance amongst N. gonorrhoeae isolates is on the rise in New Delhi. Periodic monitoring of antimicrobial susceptibility pattern of N. gonorrhoeae to antimicrobials other than quinolones is essential to prevent treatment failure in patients with gonorrhoea.
推荐使用氟喹诺酮类药物治疗淋病。然而，最近有报道称氟喹诺酮类药物的敏感性降低且治疗失败。我们对2000年4月至11月连续分离出的36株淋病奈瑟菌的抗生素敏感性模式和喹诺酮耐药率进行了研究。
采用 Kirby Bauer 纸片扩散法进行抗生素敏感性试验，并用琼脂稀释法测定环丙沙星的最低抑菌浓度（MIC）。使用硝基头孢菌素纸片法鉴定产青霉素酶淋病奈瑟菌（PPNG）。
对从44例连续男性患者（81.9%）中获得的36株淋病奈瑟菌进行了研究。通过纸片扩散法，这些分离株中只有3株（8.3%）对环丙沙星敏感。所有分离株对头孢曲松均敏感，23株（63.9%）对四环素敏感，12株（33.3%）对青霉素敏感。4株（11.1%）淋病奈瑟菌分离株为PPNG。通过MIC测定发现27株（75%）分离株对环丙沙星耐药。
在新德里，淋病奈瑟菌分离株中环丙沙星耐药率呈上升趋势。定期监测淋病奈瑟菌对喹诺酮类以外抗菌药物的敏感性模式对于预防淋病患者治疗失败至关重要。
