Branchet M C, Boisnic S, Blétry O, Robert L, Charron D, Francès C
Department of Internal Medicine, Hôpital Pitié, Paris, France.
Br J Dermatol. 1992 May;126(5):431-5.
Skin biopsies were taken from 11 patients with morphoea, nine with acrosclerosis and 10 with diffuse systemic sclerosis and processed for immunohistochemical studies using a panel of monoclonal antibodies including antibodies to MHC class II antigens. A significantly higher percentage of HLA-DR positive dermal cells were observed in the reticular dermis in biopsies from patients with morphoea (44.1 +/- 16.2%), acrosclerosis (15.9 +/- 5.4%) and systemic sclerosis (39.5 +/- 2.3%) when compared with the controls (6.6 +/- 2%). A smaller percentage of dermal cells also expressed HLA-DP and -DQ. The degree of monocnuclear cell infiltrate in the biopsies, however, did not correlate with the percentage of HLA class II positive fibroblasts. In organ culture, the expression of the HLA class II antigens was almost totally lost after 3 days and was no longer detected on fibroblasts after 3 weeks of culture.
对11例硬斑病患者、9例肢端硬化症患者和10例弥漫性系统性硬化症患者进行皮肤活检，并使用一组单克隆抗体（包括针对MHC II类抗原的抗体）进行免疫组织化学研究。与对照组（6.6±2%）相比，硬斑病患者（44.1±16.2%）、肢端硬化症患者（15.9±5.4%）和系统性硬化症患者活检标本的网状真皮中，HLA-DR阳性真皮细胞的百分比显著更高。较小比例的真皮细胞也表达HLA-DP和-DQ。然而，活检标本中的单核细胞浸润程度与HLA II类阳性成纤维细胞的百分比无关。在器官培养中，HLA II类抗原的表达在3天后几乎完全丧失，培养3周后在成纤维细胞上不再检测到。
