Zhou Yong-Li, Pan Ya-Jiao, Zhai Wen-Xue, Xu Jian-Long, Zhang Qi, Li Zhi-Kang
Key Laboratory of Crop Genetic and Breeding of Ministry of Agriculture, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
Sheng Wu Gong Cheng Xue Bao. 2005 May;21(3):486-8.
The mutant population of Xanthomonas oryzae pv oryzae strain differential to rice bacterial blight resistance gene Xa23 has been constructed mediated by transposon in vivo . The results of PCR amplification with specific primers and analysis of flanking sequence of mutants indicated that the foreign DNA has been integrated into X. oryzae pv oryzae genome. Four mutants with changed avirulent activity to Xa23 gene have been identified by artificial inoculation. It is possible to clone genes that are required for AvrXa23 avirulence activity using this new strategy.
利用转座子在体内介导构建了与水稻白叶枯病抗性基因Xa23具有差异的水稻白叶枯病菌突变群体。用特异性引物进行PCR扩增及对突变体侧翼序列分析的结果表明，外源DNA已整合到水稻白叶枯病菌基因组中。通过人工接种鉴定出4个对Xa23基因无毒活性发生改变的突变体。利用这一新策略有可能克隆出AvrXa23无毒活性所需的基因。
