Sun Jie, Li Yuan-Li, Wang Ruo-Hai, Xia Gui-Xian
Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080, China.
Sheng Wu Gong Cheng Xue Bao. 2004 Jan;20(1):39-42.
Fluorescence differential display (FDD) technique was used to identify genes that are specifically or preferentially expressed in different developmental stages of cotton fiber cells. One hundred and nine differentially displayed cDNA fragments were isolated using 9, 21 and 27 DPA (days postanthesis) fibers as experimental materials. By a combination of two rounds of reverse Northern hybridization and Northern blot analyses, a number of such cDNA fragments were proved to represent fiber-specific/preferential genes. Sequencing determination and database searching indicated that most of these genes are novel. This work is an important step towards cloning the full-length cDNAs and characterizing the cellular functions of aforementioned genes in fiber development.
荧光差异显示（FDD）技术被用于鉴定在棉纤维细胞不同发育阶段特异性或优先表达的基因。以开花后9天、21天和27天的纤维作为实验材料，分离出了109个差异显示的cDNA片段。通过两轮反向Northern杂交和Northern印迹分析相结合，证实了许多此类cDNA片段代表纤维特异性/优先表达的基因。测序测定和数据库搜索表明，这些基因中的大多数是新基因。这项工作是朝着克隆上述基因的全长cDNA并表征其在纤维发育中的细胞功能迈出的重要一步。
