果蝇（Drosophila virilis）甘油-3-磷酸脱氢酶基因的结构及其与黑腹果蝇（Drosophila melanogaster）基因的比较。

Structure of Drosophila virilis glycerol-3-phosphate dehydrogenase gene and a comparison with the Drosophila melanogaster gene.

作者信息

Tominaga H, Shiba T, Narise S

机构信息

Biological Laboratory, Faculty of Science, Josai University, Saitama, Japan.

出版信息

Biochim Biophys Acta. 1992 Jun 15;1131(2):233-8. doi: 10.1016/0167-4781(92)90086-f.

DOI:10.1016/0167-4781(92)90086-f

PMID:1610907

Abstract

The complete glycerol-3-phosphate dehydrogenase gene of Drosophila virilis isolated by screening with alpha GPDHM cDNA of the adult fly was sequenced. The gene contains eight exons spread over a total of approximate 8 kb DNA. Its exon/intron organization is identical to that of D. melanogaster. A single transcription initiation site was determined by primer extension. The stop codons are located at the 3' end of each of the exons 6 to 8. TATA and CAAT boxes are present upstream of the transcriptional start site. Adult alpha GPDH protein is encoded by exons 1 to 6 and exon 8. Comparison of the sequence with that of D. melanogaster showed that the homology of the nucleotide sequence of the coding region is 85% and that the homology of the amino acid sequence is 98%. On the contrary, the non-coding region is quite different in length and nucleotide sequence.

摘要

通过用成年果蝇的αGPDHM cDNA进行筛选分离出了果蝇的完整甘油-3-磷酸脱氢酶基因，并对其进行了测序。该基因包含八个外显子，分布在总共约8 kb的DNA上。其外显子/内含子结构与黑腹果蝇相同。通过引物延伸确定了一个单一的转录起始位点。终止密码子位于外显子6至8的每个3'末端。TATA盒和CAAT盒存在于转录起始位点的上游。成年αGPDH蛋白由外显子1至6和外显子8编码。将该序列与黑腹果蝇的序列进行比较，结果表明编码区核苷酸序列的同源性为85%，氨基酸序列的同源性为98%。相反，非编码区在长度和核苷酸序列上有很大差异。