Kim Mee Kum, Heo Jang Won, Lee Jae Lim, Wee Won Ryang, Lee Jin Hak
Department of Ophthalmology, Seoul National University College of Medicine, and Artificial Eye Laboratory Clinical Research Center, Seoul National University Hospital, Seoul, Korea.
Curr Eye Res. 2005 Aug;30(8):639-46. doi: 10.1080/02713680590968277.
To investigate adhesion complex formation in cultivated human limbal epithelium after transplantation into the limbal deficient model.
Cultivated epithelium on amniotic membrane was transplanted into limbal deficient rabbits. The transplanted rabbits and the controls were sacrificed at 1, 2, 3, and 4 weeks. The adhesion complex was examined by electron microscopy and immunohistochemistry.
Morphologically identifiable hemidesmosomes appeared at 1 week, and matured adhesion complex was found at 3 weeks. Collagen VII was partly stained after transplantation. The mean numbers of hemidesmosomes/2.25 microm were 2.3 +/- 0.9, 2.5 +/- 0.5, 5.2 +/- 1.0, and 4.0 +/- 0.9 at 1, 2, 3, and 4 weeks, and all they were smaller than those in the control, respectively (p < 0.05). It reached 137.4% of the density of hemidesmosomes in human cornea at 3 weeks. The average depths of anchoring fibril were 0.10 +/- 0.03, 0.27 +/- 0.06, 0.45 +/- 0.06, and 0.46 +/- 0.12 microm at 1, 2, 3, and 4 weeks, reaching 75.0% of that in the human cornea after 3 weeks, although they were shallower than that of the control, respectively (p < 0.05).
Assembly of adhesion complex in cultivated epithelium transplanted in limbal deficient rabbit might recover to the level of that in the human after 3 weeks, although it was delayed compared with that in normal wound healing of the rabbit.
研究培养的人角膜缘上皮移植到角膜缘缺陷模型后黏附复合体的形成情况。
将羊膜上培养的上皮移植到角膜缘缺陷的兔眼。在1、2、3和4周时处死移植兔和对照兔。通过电子显微镜和免疫组织化学检查黏附复合体。
1周时出现形态学上可识别的半桥粒，3周时发现成熟的黏附复合体。移植后Ⅶ型胶原部分染色。1、2、3和4周时每2.25微米半桥粒的平均数量分别为2.3±0.9、2.5±0.5、5.2±1.0和4.0±0.9，均分别小于对照组（p<0.05）。3周时达到人角膜半桥粒密度的137.4%。1、2、3和4周时锚原纤维的平均深度分别为0.10±0.03、0.27±0.06、0.45±0.06和0.46±0.12微米，3周后达到人角膜的75.0%，尽管分别比对照组浅（p<0.05）。
移植到角膜缘缺陷兔眼中的培养上皮中黏附复合体的组装可能在3周后恢复到人类的水平，尽管与兔正常伤口愈合相比有所延迟。
