Soler C, de Monserrat J J, Nunez M, Gutierrez R, Nunez J, Sancho M, Cooper T G
Department of Functional Biology and Physical Anthropology, Universitat de Valencia, C/- Dr Moliner 50, 46100 Burjassot, Spain.
Asian J Androl. 2005 Sep;7(3):267-75. doi: 10.1111/j.1745-7262.2005.00042.x.
To establish a rat and mouse epididymal map based on the use of the Epiquatre automatic software for histologic image analysis.
Epididymides from five adult rats and five adult mice were fixed in alcoholic Bouin's fixative and embedded in paraffin. Serial longitudinal sections through the medial aspect of the organ were cut at 10 microm and stained with hematoxylin and eosin. As determined from major connective tissue septa, nine subdivisions of the rat epididymis and seven for the mouse were determined, consisting of five sub-regions in the caput (rat and mouse), one (mouse) or three (rat) in the corpus and one in the cauda (rat and mouse). Using the Epiquatre software, several tubular, luminal and epithelial morphometric parameters were evaluated.
Statistical comparison of the quantitative parameters revealed regional differences (2-5 in the rat, 3-6 in the mouse, dependent on parameters) with caput regions 1 and 2 being largely distinguishable from the similar remaining caput and corpus, which were in turn recognizable from the cauda regions in both species.
The use of the Epiquatre software allowed us to establish regression curves for different morphometric parameters that can permit the detection of changes in their values under different pathological or experimental conditions.
基于使用Epiquatre自动软件进行组织学图像分析，建立大鼠和小鼠附睾图谱。
将五只成年大鼠和五只成年小鼠的附睾用酒精波因氏固定液固定，石蜡包埋。从器官内侧制作连续的纵切片，厚度为10微米，并用苏木精和伊红染色。根据主要结缔组织间隔确定，大鼠附睾分为九个亚区，小鼠附睾分为七个亚区，包括头部的五个亚区（大鼠和小鼠）、体部的一个亚区（小鼠）或三个亚区（大鼠）以及尾部的一个亚区（大鼠和小鼠）。使用Epiquatre软件评估了几个管状、管腔和上皮形态计量学参数。
定量参数的统计比较显示存在区域差异（大鼠为2至5个，小鼠为3至6个，取决于参数），头部的1区和2区与其余相似的头部和体部有很大区别，而这又与两个物种的尾部区域可区分。
使用Epiquatre软件使我们能够建立不同形态计量学参数的回归曲线，从而能够检测在不同病理或实验条件下其值的变化。
