Muhlmann Maria C, Laudicina Alejandro O, Perandones Claudia, Bertolino Maria V, Marazzi Andrea, Quintans Carlos J, Donaldson Monica, Bozzo Walter, Pasqualini Sergio
Laboratorio de Citogenética Molecular, Radiobiología, CAC-CONICET, Argentina.
Genet Mol Res. 2005 Jun 30;4(2):143-51.
We studied chromosomal abnormalities in arrested embryos produced by assisted reproductive technology with fluorescence in situ hybridization (FISH) and comparative genomic hybridization (CGH) in order to determine the best technique for evaluating chromosomal aneusomies to be implemented in different situations. We examined individual blastomeres from arrested embryos by FISH and arrested whole embryos by CGH. All of the 10 FISH-analyzed embryos gave results, while only 7 of the 30 embryos analyzed by CGH were usable. Fifteen of the 17 embryos were chromosomally abnormal. CGH provided more accurate data for arrested embryos; however, FISH is the technique of choice for screening in preimplantation genetic diagnosis, because the results can be obtained within a day, while the embryos are still in culture.
我们运用荧光原位杂交（FISH）和比较基因组杂交（CGH）技术，研究辅助生殖技术产生的停滞胚胎中的染色体异常情况，以确定在不同情况下评估染色体非整倍体的最佳技术。我们通过FISH检测停滞胚胎的单个卵裂球，通过CGH检测停滞的完整胚胎。所有10个经FISH分析的胚胎都得出了结果，而经CGH分析的30个胚胎中只有7个可用。17个胚胎中有15个存在染色体异常。CGH为停滞胚胎提供了更准确的数据；然而，FISH是植入前基因诊断筛查的首选技术，因为可以在一天内获得结果，此时胚胎仍在培养中。
