Fu Chong-Luo, Song Wei-Bo, Li Yun, Zhang Yong-Zhong, Yan Hua-Chao
Key Laboratory of Mariculture, Ministry of Education, Ocean University of China, Qingdao 266003, China.
Wei Sheng Wu Xue Bao. 2004 Dec;44(6):741-4.
The naturally infected scallops Chlamys farreri sampled during mass mortality in summer of 2003 was detected by means of histopathological and MAb-based immunofluorescence assay (IFA). The results of histological examination demonstrated that a series of histopathological changes including cell swelling, basophilic increase, disorder, partial sloughing and excessive sloughing were always observed in epithelia of many different organs, e.g. mantle, gills, stomach, intestine and kidney. Additionally, the infected tissues were applied for in situ detection of the "acute virus necrobiotic disease" (AVND) virus by means of specific MAb-based IFA, and the result demonstrated that this pathological changes or lesions were perfectly coincident with the positive cells (fluorescencing cells) . The positive cells were denser in some local area of epithelia, and exhibited serious pathological lesions, which would reveal the roles of this virus in pathogenesis and further confirm that the AVND virus is the main causative agent of mass mortalities among cultured scallop Chlamys farreri farmed in northern coast of China.
采用组织病理学和基于单克隆抗体的免疫荧光检测法（IFA）对2003年夏季大规模死亡期间自然感染的栉孔扇贝进行检测。组织学检查结果表明，在许多不同器官如外套膜、鳃、胃、肠和肾的上皮中总是观察到一系列组织病理学变化，包括细胞肿胀、嗜碱性增强、紊乱、部分脱落和过度脱落。此外，通过基于特异性单克隆抗体的IFA对感染组织进行“急性病毒性坏死病”（AVND）病毒的原位检测，结果表明这种病理变化或病变与阳性细胞（荧光细胞）完全一致。阳性细胞在上皮的某些局部区域更密集，并表现出严重的病理损伤，这将揭示该病毒在发病机制中的作用，并进一步证实AVND病毒是中国北方养殖栉孔扇贝大规模死亡的主要病原体。
