变性梯度凝胶电泳和温度梯度凝胶电泳在微生物分子生态学中的应用
[Application of denaturing gradient gel electrophoresis and temperature gradient gel electrophoresis in microbial molecular ecology].
作者信息
Gong Man-Li, Ren Nan-Qi, Xing De-Feng
机构信息
School of Municipal and Environmental Engineering, Harbin Institute of Technology, Harbin 150090, China.
出版信息
Wei Sheng Wu Xue Bao. 2004 Dec;44(6):845-8.
PMID:16110975
Abstract
In recent years, denaturing gradient gel electrophoresis (DGGE) and temperature gradient gel electrophoresis (TGGE) are well-established molecular tools in microbial molecular ecology that allows the study of diversity and dynamics of microbial communities. The technique is reliable, reproducible rapid and inexpensive. Here, the principle, the limitations and potentials application of DGGE/TGGE techniques are introduced in this paper.
摘要
近年来，变性梯度凝胶电泳（DGGE）和温度梯度凝胶电泳（TGGE）是微生物分子生态学中成熟的分子工具，可用于研究微生物群落的多样性和动态变化。该技术可靠、可重复、快速且成本低廉。本文介绍了DGGE/TGGE技术的原理、局限性及潜在应用。
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