变性梯度凝胶电泳和温度梯度凝胶电泳在微生物分子生态学中的应用
[Application of denaturing gradient gel electrophoresis and temperature gradient gel electrophoresis in microbial molecular ecology].
作者信息
Gong Man-Li, Ren Nan-Qi, Xing De-Feng
机构信息
School of Municipal and Environmental Engineering, Harbin Institute of Technology, Harbin 150090, China.
出版信息
Wei Sheng Wu Xue Bao. 2004 Dec;44(6):845-8.
PMID:16110975
Abstract
In recent years, denaturing gradient gel electrophoresis (DGGE) and temperature gradient gel electrophoresis (TGGE) are well-established molecular tools in microbial molecular ecology that allows the study of diversity and dynamics of microbial communities. The technique is reliable, reproducible rapid and inexpensive. Here, the principle, the limitations and potentials application of DGGE/TGGE techniques are introduced in this paper.
摘要
近年来，变性梯度凝胶电泳（DGGE）和温度梯度凝胶电泳（TGGE）是微生物分子生态学中成熟的分子工具，可用于研究微生物群落的多样性和动态变化。该技术可靠、可重复、快速且成本低廉。本文介绍了DGGE/TGGE技术的原理、局限性及潜在应用。
相似文献
1
[Application of denaturing gradient gel electrophoresis and temperature gradient gel electrophoresis in microbial molecular ecology].
Wei Sheng Wu Xue Bao. 2004 Dec;44(6):845-8.
2
[Common problems in the analyses of microbial community by denaturing gradient gel electrophoresis (DGGE)].
Wei Sheng Wu Xue Bao. 2006 Apr;46(2):331-5.
3
DGGE/TGGE a method for identifying genes from natural ecosystems.
Curr Opin Microbiol. 1999 Jun;2(3):317-22. doi: 10.1016/S1369-5274(99)80055-1.
4
Denaturing gradient gel electrophoresis gel expansion (DGGEGE)--an attempt to resolve the limitations of co-migration in the DGGE of complex polymicrobial communities.
FEMS Microbiol Lett. 2005 Dec 15;253(2):303-7. doi: 10.1016/j.femsle.2005.09.048. Epub 2005 Oct 13.
5
Characterization of bacteria in mixed biofilm communities using denaturing gradient gel electrophoresis (DGGE).
Curr Protoc Microbiol. 2007 Feb;Chapter 1:Unit 1E.1. doi: 10.1002/9780471729259.mc01e01s4.
6
How to get more out of molecular fingerprints: practical tools for microbial ecology.
Environ Microbiol. 2008 Jun;10(6):1571-81. doi: 10.1111/j.1462-2920.2008.01572.x. Epub 2008 Mar 4.
7
Application of denaturing gradient gel electrophoresis (DGGE) and temperature gradient gel electrophoresis (TGGE) in microbial ecology.
Antonie Van Leeuwenhoek. 1998 Jan;73(1):127-41. doi: 10.1023/a:1000669317571.
8
PCR-DGGE fingerprinting: novel strategies for detection of microbes in food.
J Microbiol Methods. 2004 Mar;56(3):297-314. doi: 10.1016/j.mimet.2003.11.006.
9
[Analysis of bacterial diversity of kefir grains by denaturing gradient gel electrophoresis and 16S rDNA sequencing].
Wei Sheng Wu Xue Bao. 2006 Apr;46(2):310-3.
10
Direct comparison of single-strand conformation polymorphism (SSCP) and denaturing gradient gel electrophoresis (DGGE) to characterize a microbial community on the basis of 16S rRNA gene fragments.
J Microbiol Methods. 2006 Jul;66(1):165-9. doi: 10.1016/j.mimet.2005.11.007. Epub 2005 Dec 20.
Zotero 插件