Muyzer G, Smalla K
Max-Planck-Institute for Marine Microbiology, Bremen, Germany.
Antonie Van Leeuwenhoek. 1998 Jan;73(1):127-41. doi: 10.1023/a:1000669317571.
Here, the state of the art of the application of denaturing gradient gel electrophoresis (DGGE) and temperature gradient gel electrophoresis (TGGE) in microbial ecology will be presented. Furthermore, the potentials and limitations of these techniques will be discussed, and it will be indicated why their use in ecological studies has become so important.
本文将介绍变性梯度凝胶电泳(DGGE)和温度梯度凝胶电泳(TGGE)在微生物生态学中的应用现状。此外,还将讨论这些技术的潜力和局限性,并说明它们在生态学研究中应用如此重要的原因。