van der Harst Mark R, DeGroot Jeroen, Kiers Geesje H, Brama Pieter A J, van de Lest Chris H A, van Weeren P René
Department of Equine Sciences, Faculty of Veterinary Medicine, Utrecht University, Yalelaan 12, 3584 CM Utrecht, The Netherlands.
Am J Vet Res. 2005 Jul;66(7):1238-46. doi: 10.2460/ajvr.2005.66.1238.
To assess whether site-related changes in biochemical composition are present in the cartilage and subchondral and trabecular bone of the metacarpophalangeal joint of horses with early osteoarthritis.
Right metacarpophalangeal joints from 59 mature warmblood horses.
Biochemical data (cross-link, amino acid, DNA, and ash contents; denatured collagen and glycosaminoglycan [GAG] concentrations; bone mineral density; and mineral composition) were obtained from 2 differently loaded sites of phalanx I cartilage and subchondral and trabecular bone samples; data were compared with previously published values from nonosteoarthritic equine joints.
Compared with findings in nonosteoarthritic joints, GAG concentration was lower in cartilage from osteoarthritic joints and there was a loss of site differences in cellularity and lysylpyridinoline (LP) cross-link content. In subchondral bone, LP cross-link content was decreased overall and there was a loss of site differences in osteoarthritic joints; ash content was higher in the osteoarthritic joints. Hydroxyproline content in trabecular bone from osteoarthritic joints was greater than that in nonosteoarthritic trabecular bone. In all 3 layers and at both sites, the linear increase of the pentosidine cross-link content with age had diminished or was not apparent in the horses with osteoarthritic joints.
In equine metacarpophalangeal joints with early osteoarthritis, distinct biochemical changes were detected in the cartilage and subchondral and trabecular bone. The dissimilarity in response of the different tissues and differences between the sites that are affected may be related to differences in biomechanical loading and transmission and dissipation of force.
评估患有早期骨关节炎的马的掌指关节软骨、软骨下骨和小梁骨中是否存在与部位相关的生化成分变化。
59匹成熟温血马的右掌指关节。
从第一指骨软骨、软骨下骨和小梁骨样本的2个不同受力部位获取生化数据（交联、氨基酸、DNA和灰分含量；变性胶原蛋白和糖胺聚糖[GAG]浓度；骨矿物质密度；以及矿物质成分）；将数据与先前发表的非骨关节炎马关节的值进行比较。
与非骨关节炎关节的结果相比，骨关节炎关节软骨中的GAG浓度较低，细胞密度和赖氨酰吡啶啉（LP）交联含量的部位差异消失。在软骨下骨中，LP交联含量总体下降，骨关节炎关节中的部位差异消失；骨关节炎关节的灰分含量较高。骨关节炎关节小梁骨中的羟脯氨酸含量高于非骨关节炎小梁骨。在所有3层和两个部位，患有骨关节炎关节的马中，戊糖苷交联含量随年龄的线性增加减弱或不明显。
在患有早期骨关节炎的马掌指关节中，在软骨、软骨下骨和小梁骨中检测到明显的生化变化。不同组织反应的差异以及受影响部位之间的差异可能与生物力学负荷以及力的传递和消散差异有关。
