1α,25 - 二羟基维生素D3和12 - O - 十四烷酰佛波醇 - 13 - 乙酸酯对人HL60白血病细胞诱导的免疫表型变化。
Immunophenotypic changes induced on human HL60 leukaemia cells by 1alpha,25-dihydroxyvitamin D3 and 12-O-tetradecanoyl phorbol-13-acetate.
作者信息
White Sarah L, Belov Larissa, Barber Nicole, Hodgkin Philip D, Christopherson Richard I
机构信息
School of Molecular and Microbial Biosciences, University of Sydney, NSW 2006, Australia.
出版信息
Leuk Res. 2005 Oct;29(10):1141-51. doi: 10.1016/j.leukres.2005.02.012. Epub 2005 Mar 17.
1alpha,25-Dihydroxyvitamin D3 (1,25D3) induces HL60 cells to acquire a monocyte-like phenotype, while cells treated with 12-O-tetradecanoyl phorbol-13-acetate (TPA) resemble macrophages. Using a microarray of 82 CD antibodies, 24 cluster of differentiation (CD) antigens were detected on HL60 cells. 1,25D3 induced the following antigens in decreasing order of the change: CD14, CD11c, CD11b, CD54, CD86, CD38 and CD66c, with repression of CD117, CD71, CD95, CD45 and CD64. TPA induced the following antigens in decreasing order of the change: CD11c, CD9, CD11b, CD54, CD38, CD45RO and CD66c, with repression of CD4, CD117, CD95, CD71 and CD64. The results presented provide a basis for monitoring differentiation therapy of myeloid leukaemias in patients.
1α,25-二羟基维生素D3（1,25D3）诱导HL60细胞获得单核细胞样表型，而用12-O-十四烷酰佛波醇-13-乙酸酯（TPA）处理的细胞类似于巨噬细胞。使用包含82种CD抗体的微阵列，在HL60细胞上检测到24种分化簇（CD）抗原。1,25D3诱导的抗原变化顺序递减如下：CD14、CD11c、CD11b、CD54、CD86、CD38和CD66c，同时CD117、CD71、CD95、CD45和CD64表达受抑制。TPA诱导的抗原变化顺序递减如下：CD11c、CD9、CD11b、CD54、CD38、CD45RO和CD66c，同时CD4、CD117、CD95、CD71和CD64表达受抑制。所呈现的结果为监测患者髓系白血病的分化治疗提供了依据。
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