Delayed type hypersensitivity (DTH): studies on necrotising DTH-reactions against listerial antigen in the skin of guinea pigs.

Delayed type hypersensitivity (DTH) to facultative intracellular bacteria which leads to destructive skin reactions was so far only investigated against mycobacterial antigens in guinea pigs whereas this work investigates destructive DTH-reactions in the skin of guinea pigs which are directed against listerial antigens. Toxic factors of viable listerias induced an enhancement of destructive skin reactions in non-immunised guinea pigs as compared to immunised ones. In contrast, heat killed listerias (HKL) induced necrotising skin reactions in immunised and non-immunised guinea pigs which were significantly enhanced by DTH in immunised guinea pigs. 5 days after immunisation, necrotising reactivity was maximal and increased in a dose-dependent mode with higher amounts of HKL. Listeria-specific T-cells were able to interact specifically with allogeneic macrophages in vitro. By means of adoptive transfer of listeria-specific T-cells it was possible to transfer successfully Listeria-specific DTH-reactivity from immunised donors to non-immunised recipients.