Koga T, Mitsuyama M, Handa T, Yayama T, Muramori K, Nomoto K
Department of Immunology, Kyushu University, Fukuoka, Japan.
Immunology. 1987 Oct;62(2):241-8.
Using a local passive transfer system, we found that effector T cells mediating delayed-type hypersensitivity (DTH) but not acquired cellular resistance (ACR) to Listeria monocytogenes (strain EGD) were generated in mice immunized with killed Listeria, although immunized mice did not express DTH or ACR. When non-adherent cells of peritoneal, lymph node, or spleen cells from mice immunized with killed Listeria were transferred into the footpad of naive recipient mice along with eliciting antigen, positive delayed footpad reaction (DFR) was elicited. However, there was no evident protection against challenge at the site of the local transfer. Cells from mice immunized with viable Listeria conferred significant degrees of DFR and ACR on the recipients. DFR transferred by cells immunized with killed Listeria was mediated by L3T4+ T cells in an antigen-specific manner. The antigen-specific proliferative response of T cells from mice immunized with killed Listeria was much lower than that of T cells from mice immunized with viable Listeria. The production of macrophage chemotactic factor (MCF) by cells from killed Listeria-immune mice was much the same as that by cells from viable Listeria-immune mice. In contrast, the production of interleukin-2 (IL-2) and macrophage activating factor (MAF) was much lower in cells from killed Listeria-immune mice. The elimination of L. monocytogenes (strain L461), a strain of low virulence, was enhanced at the site of DFR transferred with cells from killed Listeria-immune mice. These results suggest that stimulation with killed bacteria is effective for the generation of DTH-mediating effector T cells, and that different effector T cells mediating DTH or ACR are involved in cell-mediated immunity to L. monocytogenes.
使用局部被动转移系统，我们发现，在用灭活李斯特菌免疫的小鼠中可产生介导迟发型超敏反应（DTH）但不介导对单核细胞增生李斯特菌（菌株EGD）获得性细胞抗性（ACR）的效应T细胞，尽管免疫小鼠并未表现出DTH或ACR。当将用灭活李斯特菌免疫的小鼠的腹膜、淋巴结或脾细胞中的非黏附细胞与引发抗原一起转移到未免疫受体小鼠的足垫中时，可引发阳性迟发型足垫反应（DFR）。然而，在局部转移部位对攻击并没有明显的保护作用。用活李斯特菌免疫的小鼠的细胞可使受体产生显著程度的DFR和ACR。用灭活李斯特菌免疫的细胞转移的DFR由L3T4 + T细胞以抗原特异性方式介导。用灭活李斯特菌免疫的小鼠的T细胞的抗原特异性增殖反应远低于用活李斯特菌免疫的小鼠的T细胞。用灭活李斯特菌免疫的小鼠的细胞产生巨噬细胞趋化因子（MCF）的情况与用活李斯特菌免疫的小鼠的细胞大致相同。相比之下，用灭活李斯特菌免疫的小鼠的细胞中白细胞介素-2（IL-2）和巨噬细胞激活因子（MAF）的产生要低得多。在用灭活李斯特菌免疫的小鼠的细胞转移的DFR部位，低毒力菌株单核细胞增生李斯特菌（菌株L461）的清除得到增强。这些结果表明，用灭活细菌刺激对于产生介导DTH的效应T细胞是有效的，并且介导DTH或ACR的不同效应T细胞参与了对单核细胞增生李斯特菌的细胞介导免疫。
