Choi Sang-Ho, Im Eunok, Kang Hyun Kyung, Lee Ji-Hyeon, Kwak Hi-Suk, Bae Young-Tae, Park Hee-Juhn, Kim Nam Deuk
Department of Pharmacy, College of Pharmacy, and Research Institute for Drug Development, Pusan National University, Busan 609-735, South Korea.
Cancer Lett. 2005 Sep 28;227(2):153-62. doi: 10.1016/j.canlet.2005.01.011.
Costunolide, a natural sesquiterpene compound, has been known having cytotoxic and chemopreventive effects on various human cancer cells. In the present study, we examined the effects of costunolide on telomerase activity and on the components of telomerase in MCF-7 (wild-type p53) and MDA-MB-231 (mutant p53) cells. We found that costunolide inhibited the growth and telomerase activity of MCF-7 and MDA-MB-231 cells in a concentration- and time-dependent manner. The expression of hTERT mRNA was also inhibited but hTR mRNA was not. In addition, the bindings of transcription factors in hTERT promoters were significantly decreased in both cells by the treatment of costunolide. These results suggest that costunolide inhibited the growth of both MCF-7 and MDA-MB-231 cells and this effect was mediated at least in part by a significant reduction in telomerase activity.
木香烯内酯是一种天然倍半萜化合物，已知其对多种人类癌细胞具有细胞毒性和化学预防作用。在本研究中，我们检测了木香烯内酯对MCF-7（野生型p53）和MDA-MB-231（突变型p53）细胞中端粒酶活性及端粒酶组分的影响。我们发现木香烯内酯以浓度和时间依赖性方式抑制MCF-7和MDA-MB-231细胞的生长及端粒酶活性。hTERT mRNA的表达也受到抑制，但hTR mRNA未受影响。此外，经木香烯内酯处理后，两种细胞中hTERT启动子上转录因子的结合显著减少。这些结果表明，木香烯内酯抑制MCF-7和MDA-MB-231细胞的生长，且这种作用至少部分是由端粒酶活性的显著降低介导的。
