Guinn Barbara-Ann, Bland Elizabeth A, Lodi Usman, Liggins Amanda P, Tobal Khalid, Petters Sarah, Wells James W, Banham Alison H, Mufti Ghulam J
Department of Haematological Medicine, Guy's, King's and St. Thomas' School of Medicine, King's College London, The Rayne Institute, 123 Coldharbour Lane, London SE5 9NU, UK.
Biochem Biophys Res Commun. 2005 Oct 7;335(4):1293-304. doi: 10.1016/j.bbrc.2005.08.024.
The serological analysis of recombinant cDNA expression libraries (SEREX) technique was used to immunoscreen a testes cDNA expression library with sera from newly diagnosed acute myeloid leukaemia (AML) patients. We used a testis cDNA library to aid our identification of cancer-testis (CT) antigens. We identified 44 antigens which we further immunoscreened with sera from AML, chronic myeloid leukaemia (CML), and normal donors. Eight antigens were solely recognised by patient sera including the recently described CT antigen, PASD1, and the cancer-related SSX2 interacting protein, SSX2IP. RT-PCR analysis indicated that we had identified three antigens which were expressed in patient bone marrow (BM) and peripheral blood (PB) but not in normal donor samples (PASD1, SSX2IP, and GRINL1A). Real-time PCR (RQ-PCR) confirmed the restricted expression of PASD1 in normal donor organs. Antigen presentation assays using monocyte-derived dendritic cells (mo-DCs) showed that PASD1 could stimulate autologous T-cell responses, suggesting that PASD1 could be a promising target for future immunotherapy clinical trials.
采用重组cDNA表达文库血清学分析（SEREX）技术，用新诊断的急性髓系白血病（AML）患者的血清对睾丸cDNA表达文库进行免疫筛选。我们利用睾丸cDNA文库来帮助鉴定癌-睾丸（CT）抗原。我们鉴定出44种抗原，并用AML、慢性髓系白血病（CML）患者及正常供者的血清对其进一步进行免疫筛选。8种抗原仅被患者血清识别，包括最近描述的CT抗原PASD1以及与癌症相关的SSX2相互作用蛋白SSX2IP。逆转录-聚合酶链反应（RT-PCR）分析表明，我们鉴定出了3种在患者骨髓（BM）和外周血（PB）中表达但在正常供者样本中不表达的抗原（PASD1、SSX2IP和GRINL1A）。实时定量聚合酶链反应（RQ-PCR）证实了PASD1在正常供者器官中的限制性表达。使用单核细胞来源的树突状细胞（mo-DC）进行的抗原呈递试验表明，PASD1可刺激自体T细胞反应，这表明PASD1可能是未来免疫治疗临床试验的一个有前景的靶点。
