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Detection of Brazilian spotted fever infection by polymerase chain reaction in a patient from the state of São Paulo.

作者信息

Nascimento Elvira Maria Mendes, Gehrke Flávia de Sousa, Maldonado Rosa Amélia, Colombo Silvia, Silva Luiz Jacintho da, Schumaker Teresinha Tizu Sato

机构信息

Laboratório de Rickettsias, Instituto Adolfo Lutz, São Paulo, SP, Brazil.

出版信息

Mem Inst Oswaldo Cruz. 2005 May;100(3):277-9. doi: 10.1590/s0074-02762005000300011. Epub 2005 Aug 15.

DOI:10.1590/s0074-02762005000300011
PMID:16113868
Abstract

Brazilian spotted fever (BSF) cases have been increasing in the state of São Paulo but no genomic information about local rickettsia isolated from humans has been well documented. We recovered spotted-fever group rickettsiae from a sample of patient blood cultured in Vero cells using the shell vial technique. Rickettsial DNA fragments (gltA, ompA, and, ompB genes) were detected, and analysis of the ompB gene base sequences showed identity with the Rickettsia rickettsii ompB sequence available in the GenBank.

摘要

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