在巴西圣保罗州不同城市的24名患者的血凝块中检测到立氏立克次体和立克次体属。

Detection of Rickettsia rickettsii and Rickettsia sp. in blood clots in 24 patients from different municipalities of the State of Sao Paulo, Brazil.

作者信息

Gehrke Flávia Sousa, Mendes do Nascimento Elvira Maria, Rodrigues de Souza Eliana, Colombo Silvia, Jacintho da Silva Luiz, Schumaker Teresinha Tizu Sato

机构信息

Instituto de Ciências Biomédicas II, Av. Prof. Lineu Prestes, 1374, Cidade Universtitária, 05508-900 São Paulo, Brazil.

出版信息

Ann N Y Acad Sci. 2006 Oct;1078:260-2. doi: 10.1196/annals.1374.047.

DOI:10.1196/annals.1374.047

PMID:17114719

Abstract

The authors detected Rickettsia genus organisms using shell vial and polymerase chain reaction (PCR)/sequencing analysis in blood clots in patients suspected of having Brazilian spotted fever (BSF). DNA was detected using PCR with three sets of primers to access the gltA, ompA, and ompB genes. Sequence analysis was carried out using an automatic sequencer with Bioedit software. Seventy-five percent of the culture samples were positive and all samples amplified rickettsial gene fragments. To date, 46% of the samples have been sequenced.

摘要

作者们在疑似患有巴西斑疹热（BSF）的患者血凝块中，使用细胞培养瓶和聚合酶链反应（PCR）/测序分析检测立克次氏体属微生物。使用针对gltA、ompA和ompB基因的三组引物通过PCR检测DNA。使用配备Bioedit软件的自动测序仪进行序列分析。75%的培养样本呈阳性，所有样本均扩增出立克次氏体基因片段。迄今为止，46%的样本已进行测序。