Wang Jian, Liu Yun-sheng
Departnent of Neurosurgery, Second Affiated Hospital of Guangzhou Medical College, Guangzhou 510260, China.
Zhong Nan Da Xue Xue Bao Yi Xue Ban. 2004 Dec;29(6):647-50.
To detect the expression of MMP-2, MMP-9 and TIMP-2 in pituitary tumors and to explore the relationship among the expressions of MMP-2, MMP-9, TIMP-2 and invasiveness of pituitary adenomas.
The expressions of MMP-2, MMP-9 and TIMP-2 were determined in invasive pituitary adenomas (n=20) and non-invasive pituitary adenomas (n=10) by using immunohistochemical method and comparative reverse transcription-polymerase chain reaction (RT-PCR). The correlation between expressions of MMP-2, MMP-9, TIMP-2 and tumor invasiveness were studied.
Compared with the non-invasive pituitary, expressions of MMP-9 significantly increased (P < 0.01). TIMP-2 expression decreased in invasive pituitary adenomas (P < 0.01). There was a reverse correlation between MMP-2 and TIMP-2 at mRNA level.
Invasive pituitary tumors are related to the high expression of MMPs and low expression of TIMP-2, suggesting that the expression of MMA-9 and TIMP-2 can be used as an index of evaluating invasive pituitary tumors.
检测垂体肿瘤中基质金属蛋白酶-2（MMP-2）、基质金属蛋白酶-9（MMP-9）和金属蛋白酶组织抑制因子-2（TIMP-2）的表达，探讨MMP-2、MMP-9、TIMP-2的表达与垂体腺瘤侵袭性之间的关系。
采用免疫组织化学方法及比较逆转录-聚合酶链反应（RT-PCR），检测20例侵袭性垂体腺瘤和10例非侵袭性垂体腺瘤中MMP-2、MMP-9和TIMP-2的表达。研究MMP-2、MMP-9、TIMP-2的表达与肿瘤侵袭性之间的相关性。
与非侵袭性垂体相比，MMP-9的表达显著增加（P < 0.01）。侵袭性垂体腺瘤中TIMP-2表达降低（P < 0.01）。在mRNA水平上，MMP-2与TIMP-2呈负相关。
侵袭性垂体肿瘤与基质金属蛋白酶的高表达和TIMP-2的低表达有关，提示MMA-9和TIMP-2的表达可作为评估侵袭性垂体肿瘤的指标。
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