Kim Sook Heun, Franses Elias I
School of Chemical Engineering, Purdue University, 480 Stadium Mall Drive, West Lafayette, IN 47907-2100, USA.
J Colloid Interface Sci. 2006 Mar 1;295(1):84-92. doi: 10.1016/j.jcis.2005.07.056. Epub 2005 Aug 22.
The competitive adsorption of fibrinogen (FB) and DPPC at the air/aqueous interface, in phosphate buffer saline at 25 degrees C, was studied with tensiometry, infrared reflection absorption spectroscopy (IRRAS), and ellipsometry. For FB/DPPC mixtures with 750 ppm (0.075 wt%) FB and 1000 ppm (0.10 wt%) DPPC, the tension behavior was found to be similar to that of FB when alone, even with DPPC and FB being at the interface. Thus, FB interferes with adsorption of DPPC and inhibits its surface tension lowering ability. When FB protein is introduced in the solution after a DPPC monolayer has formed, the adsorption of FB is inhibited by the DPPC monolayer. When a DPPC monolayer is spread onto a solution with a preadsorbed FB layer, the DPPC monolayer excludes FB from the surface and controls the tension behavior with little inhibition by FB. When a DPPC dispersion is introduced with the Trurnit method, or sprayed dropwise, onto an aqueous FB/DPPC surfaces, the DPPC layer formed on the surface prevents the adsorption of FB and dominates the surface tension behavior. These results have implications in controlling the inhibition of lung surfactant tension behavior by serum proteins, when they leak at the alveolar lining layer, and in developing surfactant replacement therapies for alveolar respiratory diseases.
在25℃的磷酸盐缓冲盐水中，采用张力测定法、红外反射吸收光谱法（IRRAS）和椭偏仪研究了纤维蛋白原（FB）和二棕榈酰磷脂酰胆碱（DPPC）在空气/水界面的竞争吸附。对于含有750 ppm（0.075 wt%）FB和1000 ppm（0.10 wt%）DPPC的FB/DPPC混合物，即使DPPC和FB都在界面处，其张力行为也与单独的FB相似。因此，FB会干扰DPPC的吸附并抑制其降低表面张力的能力。当在DPPC单分子层形成后将FB蛋白引入溶液中时，FB的吸附会受到DPPC单分子层的抑制。当将DPPC单分子层铺展在预先吸附有FB层的溶液上时，DPPC单分子层会将FB排除在表面之外，并控制张力行为，而几乎不受FB的抑制。当采用特鲁尼特法或逐滴喷雾法将DPPC分散体引入到含水的FB/DPPC表面时，在表面形成的DPPC层会阻止FB的吸附并主导表面张力行为。这些结果对于控制血清蛋白在肺泡内衬层泄漏时对肺表面活性剂张力行为的抑制作用以及开发用于肺泡呼吸系统疾病的表面活性剂替代疗法具有重要意义。
