Ohama Yumi, Heike Yuji, Sugahara Takuya, Sakata Kozue, Yoshimura Norikazu, Hisaeda Yoshio, Hosokawa Mami, Takashima Shigemitsu, Kato Keiichi
Faculty of Agriculture, Ehime University, Japan.
Biosci Biotechnol Biochem. 2005 Aug;69(8):1453-8. doi: 10.1271/bbb.69.1453.
Lipid vesicles are potentially useful as microcapsules for drug and/or gene delivery. We developed cationic lipid vesicles consisting mainly of sorbitan monooleate (Span 80) and cationic peptide lipid (CPL), and evaluated the CPL vesicles as gene transfection vectors. The optimum CPL concentration for gene transfection into HeLa cells was found to be 20 wt % of total lipid, and such CPL vesicles did not exhibit significant cytotoxicity. Co-culture of Poly-L-lysine and plasmids prior to making CPL vesicle-plasmid complexes was effective. Lipofection using LipofectAMINE was suppressed in 10% serum-supplemented medium. The transfection efficiency of 20 wt % CPL vesicles, however, was not affected by serum in the medium when plasmids were treated with poly-L-lysine.
脂质囊泡作为药物和/或基因递送的微胶囊具有潜在的应用价值。我们开发了主要由脱水山梨醇单油酸酯（司盘80）和阳离子肽脂质（CPL）组成的阳离子脂质囊泡，并将CPL囊泡作为基因转染载体进行了评估。发现转染HeLa细胞的最佳CPL浓度为总脂质的20 wt%，且这种CPL囊泡没有表现出明显的细胞毒性。在制备CPL囊泡-质粒复合物之前，将聚-L-赖氨酸和质粒共培养是有效的。在添加10%血清的培养基中，使用LipofectAMINE进行的脂质转染受到抑制。然而，当质粒用聚-L-赖氨酸处理时，20 wt% CPL囊泡的转染效率不受培养基中血清的影响。
